Mariano Garcia-Blanco, MD, PhD

Chair

  • 9894 Citations
  • 52 h-Index
1975 …2020
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Research Output 1975 2019

  • 9894 Citations
  • 52 h-Index
  • 163 Article
  • 5 Chapter
  • 4 Review article
  • 1 Comment/debate
2003
69 Citations (Scopus)
Receptor, Fibroblast Growth Factor, Type 2
Exons
Alternative Splicing
Epithelial Cells
RNA Precursors
30 Citations (Scopus)

Asymmetric distribution of nuclear pore complexes and the cytoplasmic localization of β2-tubulin mRNA in Chlamydomonas reinhardtii

Colón-Ramos, D. A., Salisbury, J. L., Sanders, M. A., Shenoy, S. M., Singer, R. H. & Garcia-Blanco, M., Jun 1 2003, In : Developmental Cell. 4, 6, p. 941-952 12 p.

Research output: Contribution to journalArticle

Nuclear Pore
Chlamydomonas reinhardtii
Tubulin
Nuclear Pore Complex Proteins
Cell Polarity
7 Citations (Scopus)

Mending the message

Garcia-Blanco, M., Dec 2003, In : Nature Biotechnology. 21, 12, p. 1448-1449 2 p.

Research output: Contribution to journalArticle

Endoribonucleases
Gene therapy
Animals
Messenger RNA
56 Citations (Scopus)

Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing

Garcia-Blanco, M., Aug 2003, In : Journal of Clinical Investigation. 112, 4, p. 474-480 7 p.

Research output: Contribution to journalArticle

Trans-Splicing
Spliceosomes
Introns
Messenger RNA
Human Genome
25 Citations (Scopus)

Of urchins and men: Evolution of an alternative splicing unit in fibroblast growth factor receptor genes

Mistry, N., Harrington, W., Lasda, E., Wagner, E. & Garcia-Blanco, M., Feb 1 2003, In : RNA. 9, 2, p. 209-217 9 p.

Research output: Contribution to journalArticle

Fibroblast Growth Factor Receptors
Alternative Splicing
Genes
Sea Urchins
Proteome
129 Citations (Scopus)

Phenotype correction of hemophilia A mice by spliceosome-mediated RNA trans-splicing

Chao, H., Mansfield, S. G., Bartel, R. C., Hiriyanna, S., Mitchell, L. G., Garcia-Blanco, M. & Walsh, C. E., Aug 1 2003, In : Nature Medicine. 9, 8, p. 1015-1019 5 p.

Research output: Contribution to journalArticle

Trans-Splicing
Spliceosomes
Hemophilia A
RNA
Phenotype
19 Citations (Scopus)

Quantification of alternatively spliced FGFR2 RNAs using the RNA invasive cleavage assay

Wagner, E., Curtis, M. L., Robson, N. D., Baraniak, A. P., Eis, P. S. & Garcia-Blanco, M., Dec 2003, In : RNA. 9, 12, p. 1552-1561 10 p.

Research output: Contribution to journalArticle

Receptor, Fibroblast Growth Factor, Type 2
RNA Cleavage
Exons
RNA
Protein Isoforms
2002
RNA Interference
Exons
127 Citations (Scopus)

Partial correction of endogenous ΔF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing

Liu, X., Jiang, Q., Mansfield, S. G., Puttaraju, M., Zhang, Y., Zhou, W., Cohn, J. A., Garcia-Blanco, M., Mitchell, L. G. & Engelhardt, J. F., 2002, In : Nature Biotechnology. 20, 1, p. 47-52 6 p.

Research output: Contribution to journalArticle

Trans-Splicing
Spliceosomes
Cystic Fibrosis Transmembrane Conductance Regulator
RNA
Cystic Fibrosis
293 Citations (Scopus)

Reversible cross-linking combined with immunoprecipitation to study RNA-protein interactions in vivo

Niranjanakumari, S., Lasda, E., Brazas, R. & Garcia-Blanco, M., 2002, In : Methods. 26, 2, p. 182-190 9 p.

Research output: Contribution to journalArticle

Hepatitis Delta Virus
Viruses
Immunoprecipitation
RNA
Hepatitis delta Antigens
117 Citations (Scopus)

RNAi-mediated PTB depletion leads to enhanced exon definition

Wagner, E. & Garcia-Blanco, M., Oct 1 2002, In : Molecular Cell. 10, 4, p. 943-949 7 p.

Research output: Contribution to journalArticle

Polypyrimidine Tract-Binding Protein
RNA Interference
Exons
Transcriptional Silencer Elements
Protein Binding
2001
134 Citations (Scopus)

Co-transcriptional splicing of pre-messenger RNAs: Considerations for the mechanism of alternative splicing

Goldstrohm, A. C., Greenleaf, A. L. & Garcia-Blanco, M., Oct 17 2001, In : Gene. 277, 1-2, p. 31-47 17 p.

Research output: Contribution to journalArticle

RNA Splicing
RNA Splice Sites
RNA Precursors
Alternative Splicing
Introns

Erratum: Spliceosome-mediated RNA trans-splicing as a tool for gene therapy (Nature Biotechnol. (1999) 17 (246-252))

Puttaraju, M., Jamison, S. F., Mansfield, S. G., Garcia-Blanco, M. & Mitchell, L. G., 2001, In : Nature Biotechnology. 19, 3, p. 277 1 p.

Research output: Contribution to journalArticle

Gene therapy
RNA
64 Citations (Scopus)

Messenger RNA repair and restoration of protein function by spliceosome-mediated RNa trans-splicing

Puttaraju, M., DiPasquale, J., Baker, C. C., Mitchell, L. G. & Garcia-Blanco, M., 2001, In : Molecular Therapy. 4, 2, p. 105-114 10 p.

Research output: Contribution to journalArticle

Trans-Splicing
Spliceosomes
Messenger RNA
Inborn Genetic Diseases
Proteins
290 Citations (Scopus)

Minireview: Polypyrimidine tract binding protein antagonizes exon definition

Wagner, E. & Garcia-Blanco, M., 2001, In : Molecular and Cellular Biology. 21, 10, p. 3281-3288 8 p.

Research output: Contribution to journalArticle

Polypyrimidine Tract-Binding Protein
Exons
RNA-Binding Proteins
Research
96 Citations (Scopus)

The transcription elongation factor CA150 interacts with RNA polymerase II and the pre-mRNA splicing factor SF1

Goldstrohm, A. C., Albrecht, T. R., Suñé, C., Bedford, M. T. & Garcia-Blanco, M., 2001, In : Molecular and Cellular Biology. 21, 22, p. 7617-7628 12 p.

Research output: Contribution to journalArticle

Peptide Elongation Factors
RNA Polymerase II
RNA Precursors
Transcription Factors
Proline
2000
118 Citations (Scopus)
Polypyrimidine Tract-Binding Protein
Receptor, Fibroblast Growth Factor, Type 2
Exons
Protein Isoforms
Transcriptional Silencer Elements
13 Citations (Scopus)
Equine infectious anemia virus
Peptide Elongation Factors
HIV-1
Transcription Factors
Proteins
12 Citations (Scopus)

Canine cyclin T1 rescues equine infectious anemia virus tat trans- activation in human cells

Albrecht, T. R., Lund, L. H. & Garcia-Blanco, M., Mar 1 2000, In : Virology. 268, 1, p. 7-11 5 p.

Research output: Contribution to journalArticle

Cyclin T
Equine infectious anemia virus
Transcriptional Activation
Canidae
tat Gene Products
53 Citations (Scopus)

Coupled in vitro synthesis and splicing of RNA polymerase II transcripts

Ghosh, S. & Garcia-Blanco, M., 2000, In : RNA. 6, 9, p. 1325-1334 10 p.

Research output: Contribution to journalArticle

RNA Polymerase II
RNA Splicing
Ligation
Exons
In Vitro Techniques
7 Citations (Scopus)

Expression analysis and mapping of the mouse and human transcriptional regulator CA150

Bohne, J., Cole, S. E., Suñe, C., Lindman, B. R., Ko, V. D., Vogt, T. F. & Garcia-Blanco, M., 2000, In : Mammalian Genome. 11, 10, p. 930-933 4 p.

Research output: Contribution to journalArticle

Molecular Sequence Data
Amino Acid Sequence Homology
Trans-Activators
Chromosome Mapping
Amino Acid Sequence
72 Citations (Scopus)

Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase II

Carty, S. M., Goldstrohm, A. C., Suñé, C., Garcia-Blanco, M. & Greenleaf, A. L., Aug 1 2000, In : Proceedings of the National Academy of Sciences of the United States of America. 97, 16, p. 9015-9020 6 p.

Research output: Contribution to journalArticle

RNA Polymerase II
Terminal Repeat Sequences
Nuclear Proteins
Proteins
Recombinant Proteins
78 Citations (Scopus)

Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing

Mansfield, S. G., Kole, J., Puttaraju, M., Yang, C. C., Garcia-Blanco, M., Cohn, J. A. & Mitchell, L. G., 2000, In : Gene Therapy. 7, 22, p. 1885-1895 11 p.

Research output: Contribution to journalArticle

Trans-Splicing
Spliceosomes
Cystic Fibrosis Transmembrane Conductance Regulator
RNA Precursors
Messenger RNA
8 Citations (Scopus)

The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function

Lindsey-Boltz, L. A., Chawla, G., Srinivasan, N., Vijayraghavan, U. & Garcia-Blanco, M., 2000, In : RNA. 6, 9, p. 1289-1305 17 p.

Research output: Contribution to journalArticle

RNA Precursors
Amino Acids
Mutation
Transducin
RNA Splicing Factors
1999
23 Citations (Scopus)

A novel isoform ratio switch of the polypyrimidine tract binding protein

Wagner, E., Carstens, R. P. & Garcia-Blanco, M., 1999, In : Electrophoresis. 20, 4-5, p. 1082-1086 5 p.

Research output: Contribution to journalArticle

Polypyrimidine Tract-Binding Protein
Protein Isoforms
Cells
Switches
Cell Line

Erratum: Spliceosome-mediated RNA trans-splicing as a tool for gene therapy (Nature Biotechnology)

Puttaruju, M., Jamison, S. F., Mansfield, G., Garcia-Blanco, M. & Mitchell, L. G., 1999, In : Nature Biotechnology. 17, 6, p. 602 1 p.

Research output: Contribution to journalArticle

Trans-Splicing
Spliceosomes
Biotechnology
Genetic Therapy
10 Citations (Scopus)

Human immunodeficiency virus type 1 Tat-dependent activation of an arrested RNA polymerase II elongation complex

Liu, Y., Suñé, C. & Garcia-Blanco, M., Mar 15 1999, In : Virology. 255, 2, p. 337-346 10 p.

Research output: Contribution to journalArticle

RNA Polymerase II
Lac Repressors
HIV-1
tat Gene Products
Viral Genes
3 Citations (Scopus)

Prespliceosome and spliceosome isolation and analysis.

Lindsey, L. A. & Garcia-Blanco, M., 1999, In : Methods in molecular biology (Clifton, N.J.). 118, p. 351-364 14 p.

Research output: Contribution to journalArticle

Genetic Transcription
Small Nuclear Ribonucleoproteins
Spliceosomes
RNA Splicing
Macromolecular Substances
143 Citations (Scopus)

Spliceosome-mediated RNA trans-splicing as a tool for gene therapy

Puttaraju, M., Jamison, S. F., Mansfield, S. G., Garcia-Blanco, M. & Mitchell, L. G., 1999, In : Nature Biotechnology. 17, 3, p. 246-252 7 p.

Research output: Contribution to journalArticle

Trans-Splicing
Spliceosomes
Gene therapy
RNA
Genetic Therapy
48 Citations (Scopus)

Transcriptional cofactor CA150 regulates RNA polymerase II elongation in a TATA-box-dependent manner

Suñé, C. & Garcia-Blanco, M., Jul 1999, In : Molecular and Cellular Biology. 19, 7, p. 4719-4728 10 p.

Research output: Contribution to journalArticle

TATA Box
RNA Polymerase II
HIV-1
HIV Long Terminal Repeat
tat Gene Products
1998
82 Citations (Scopus)

An intronic sequence element mediates both activation and repression of rat fibroblast growth factor receptor 2 pre-mRNA splicing

Carstens, R. P., Mckeehan, W. L. & Garcia-Blanco, M., Apr 1998, In : Molecular and Cellular Biology. 18, 4, p. 2205-2217 13 p.

Research output: Contribution to journalArticle

RNA Precursors
Receptor, Fibroblast Growth Factor, Type 2
Exons
Alternative Splicing
Cell Line
39 Citations (Scopus)

A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding

Cao, W. & Garcia-Blanco, M., Aug 7 1998, In : Journal of Biological Chemistry. 273, 32, p. 20629-20635 7 p.

Research output: Contribution to journalArticle

Serine
Arginine
U1 Small Nuclear Ribonucleoproteins
Proteins
RNA Precursors
27 Citations (Scopus)

Association of chromosome 11 locus D11S12 with histology, stage, and metastases in lung cancer

Bepler, G., Fong, K. M., Johnson, B. E., O'Briant, K. C., Daly, L. A., Zimmerman, P. V., Garcia-Blanco, M. & Peterson, B., 1998, In : Cancer Detection and Prevention. 22, 1, p. 14-19 6 p.

Research output: Contribution to journalArticle

Chromosomes, Human, Pair 11
Lung Neoplasms
Histology
Alleles
Neoplasm Metastasis
18 Citations (Scopus)

Functional conservation of the human homolog of the yeast pre-mRNA splicing factor Prp17p

Lindsey, L. A. & Garcia-Blanco, M., Dec 4 1998, In : Journal of Biological Chemistry. 273, 49, p. 32771-32775 5 p.

Research output: Contribution to journalArticle

RNA Precursors
Yeast
Conservation
Yeasts
Saccharomyces cerevisiae Proteins
1997
126 Citations (Scopus)

Alternative splicing of fibroblast growth factor receptor 2 (FGF-R2) in human prostate cancer

Carstens, R. P., Eaton, J. V., Krigman, H. R., Walther, P. J. & Garcia-Blanco, M., Dec 18 1997, In : Oncogene. 15, 25, p. 3059-3065 7 p.

Research output: Contribution to journalArticle

Receptor, Fibroblast Growth Factor, Type 2
Alternative Splicing
Androgens
Prostatic Neoplasms
Heterografts
137 Citations (Scopus)

Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro

Cao, W., Jamison, S. F. & Garcia-Blanco, M., Dec 1997, In : RNA. 3, 12, p. 1456-1467 12 p.

Research output: Contribution to journalArticle

Spliceosomes
RNA Precursors
Phosphorylation
Proteins
RNA
79 Citations (Scopus)

CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription

Suñe, C., Hayashi, T., Liu, Y., Lane, W. S., Young, R. A. & Garcia-Blanco, M., Oct 1997, In : Molecular and Cellular Biology. 17, 10, p. 6029-6039 11 p.

Research output: Contribution to journalArticle

Holoenzymes
RNA Polymerase II
Nuclear Proteins
HIV-1
Transcriptional Activation
36 Citations (Scopus)

TAR RNA decoys inhibit Tat-activated HIV-1 transcription after preinitiation complex formation

Bohjanen, P. R., Liu, Y. & Garcia-Blanco, M., 1997, In : Nucleic Acids Research. 25, 22, p. 4481-4486 6 p.

Research output: Contribution to journalArticle

HIV-1
RNA
Human Immunodeficiency Virus tat Gene Products
Immobilized Nucleic Acids
Uridine Triphosphate
1996
52 Citations (Scopus)

A small circular TAR RNA decoy specifically inhibits Tat-activated HIV-1 transcription

Bohjanen, P. R., Colvin, R. A., Puttaraju, M., Been, M. D. & Garcia-Blanco, M., 1996, In : Nucleic Acids Research. 24, 19, p. 3733-3738 6 p.

Research output: Contribution to journalArticle

HIV-1
Transcriptional Activation
HIV Long Terminal Repeat
Introns
Exons
93 Citations (Scopus)

Exon circularization in mammalian nuclear extracts

Pasman, Z., Been, M. D. & Garcia-Blanco, M., 1996, In : RNA. 2, 6, p. 603-610 8 p.

Research output: Contribution to journalArticle

Exons
RNA Splice Sites
RNA
Spliceosomes
RNA Splicing
23 Citations (Scopus)

The 5′ and 3′ splice sites come together via a three dimensional diffusion mechanism

Pasman, Z. & Garcia-Blanco, M., 1996, In : Nucleic Acids Research. 24, 9, p. 1638-1645 8 p.

Research output: Contribution to journalArticle

RNA Splice Sites
Ethylene Glycol
Spliceosomes
Double-Stranded RNA
RNA Precursors
1995
21 Citations (Scopus)

A mammalian activity required for the second step of pre-messenger RNA splicing

Lindsey, L. A., Crow, A. J. & Garcia-Blanco, M., 1995, In : Journal of Biological Chemistry. 270, 22, p. 13415-13421 7 p.

Research output: Contribution to journalArticle

RNA Splicing
Yeast
Exons
Micrococcal Nuclease
Messenger RNA
35 Citations (Scopus)

Nuclear spreads: I. Visualization of bipartite ribosomal RNA domains

Garcia-Blanco, M., Miller, D. D. & Sheetz, M., 1995, In : Journal of Cell Biology. 128, 1-2, p. 15-27 13 p.

Research output: Contribution to journalArticle

Ribosomal RNA
RNA Polymerase I
RNA
Proteins
68 Citations (Scopus)
HIV Long Terminal Repeat
Sp1 Transcription Factor
terminal repeat sequences
Human immunodeficiency virus 1
HIV-1
126 Citations (Scopus)

SR proteins escort the U4/U6·U5 tri-snRNP to the spliceosome

Roscigno, R. F. & Garcia-Blanco, M., 1995, In : RNA. 1, 7, p. 692-706 15 p.

Research output: Contribution to journalArticle

Small Nuclear Ribonucleoproteins
Spliceosomes
Proteins
RNA Precursors
Recombinant Proteins
33 Citations (Scopus)
Transcription Factor TFIID
tat Gene Products
TATA-Box Binding Protein
Trans-Activators
transcriptional activation
95 Citations (Scopus)

U1 snRNP-ASF/SF2 interaction and 5′ splice site recognition: Characterization of required elements

Jamison, S. F., Pasman, Z., Wang, J., Will, C., Lührmann, R., Manley, J. L. & Garcia-Blanco, M., Aug 25 1995, In : Nucleic Acids Research. 23, 16, p. 3260-3267 8 p.

Research output: Contribution to journalArticle

U1 Small Nuclear Ribonucleoproteins
RNA Splice Sites
RNA Precursors
Messenger RNA
Interaction
1994
490 Citations (Scopus)

Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors

Kohtz, J. D., Jamison, S. F., Will, C. L., Zuo, P., Lührmann, R., Garcia-Blanco, M. & Manley, J. L., Mar 10 1994, In : Nature. 368, 6467, p. 119-124 6 p.

Research output: Contribution to journalArticle

RNA Splice Sites
RNA Precursors
U1 Small Nuclear Ribonucleoproteins
Protein Splicing
Small Nuclear Ribonucleoproteins
86 Citations (Scopus)

RNA binding by Sxl proteins in vitro and in vivo

Samuels, M. E., Bopp, D., Colvin, R. A., Roscigno, R. F., Garcia-Blanco, M. & Schedl, P., Jul 1994, In : Molecular and Cellular Biology. 14, 7, p. 4975-4990 16 p.

Research output: Contribution to journalArticle

RNA-Binding Proteins
Poly U
Proteins
Small Nuclear Ribonucleoproteins
Shock
120 Citations (Scopus)
Non-Small Cell Lung Carcinoma
Chromosomes
Loss of Heterozygosity
Wilms' Tumor Genes
Neoplasms