Role of proteolytic suPAR fragment in insulin-dependent diabetes and kidney disease

This project involves investigating the function of cleaved form of suPAR (D2D3 fragment) in pancreatic beta cells, podocytes, and tubular cells. Dr. Reiser and his lab will be responsible for:

• Performing all the pancreatic beta cell- or beta cell lines-related experiments, such as insulin secretion assay, insulin ELISA measurement, western blot, and qPCR.
• Performing all the diabetic phenotypes analysis with mouse model(s), such as the glucose stimulated insulin secretion assay (GSIS), the IF staining with pancreatic slices of WT, suPAR-Tg, and D2D3-Tg.
• Assisting in the mechanistic studies underlying suPAR and D2D3 fragment damage podocyte function.
• Assisting in the mechanistic studies underlying suPAR and D2D3 fragment damage tubular cell function.
• Assisting in the analysis of the relationships between D2D3 and renal outcomes.
• Participate in regular meetings of the research team to monitor progress of the proposed studies.
• Assisting in preparation of abstracts, manuscripts, and progress reports.