α-Fetoprotein expression in fetal kidney cells does not require enhancers

Jeffrey P. Rabek, Dora Y. Hsie, John Papaconstantinou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Expression of the α-fetoprotein (AFP) and albumin genes was examined in fetal mouse kidney by analysis of tissue mRNA pool sizes during development and transient expression assays in primary kidney tissue culture cells. AFP is expressed at a much lower level in kidney than in liver but transcription of the gene is activated early during development and repressed after birth with a time-course similar to liver. However, albumin mRNA was not detected in fetal or new born mouse kidney. Transient expression assays using AFP- and albumin-CAT (chloramphenicol acetyl transferase) vectors were employed to characterize cis-acting elements active in the regulation of AFP expression in kidney. Primary fetal liver and kidney cells in culture were used for these assays. The AFP promoter is active in kidney cells and the information necessary for tissue specific expression and developmental repression are contained within the first 1.0 kb of 5′ flanking sequences of the AFP gene. In addition, the AFP upstream enhancer elements are inactive in primary kidney cells. The mouse albumin promoter is shown to be inactive in kidney cells. The results obtained using transient expression assays are consistent with the observed low level of AFP expression, developmental repression of AFP, and the absence of expression of albumin in the mouse kidney.

Original languageEnglish (US)
Pages (from-to)317-325
Number of pages9
JournalBBA - Gene Structure and Expression
Volume1130
Issue number3
DOIs
StatePublished - Apr 6 1992

Fingerprint

Fetal Proteins
Albumins
Assays
Kidney
Liver
Genes
Tissue
Tissue culture
Messenger RNA
5' Flanking Region
Chloramphenicol
Transcription
Transferases
Cell Culture Techniques
Parturition

Keywords

  • cis-acting sequence
  • Gene regulation
  • Promoter
  • Tissue specific gene expression
  • α-Fetoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

α-Fetoprotein expression in fetal kidney cells does not require enhancers. / Rabek, Jeffrey P.; Hsie, Dora Y.; Papaconstantinou, John.

In: BBA - Gene Structure and Expression, Vol. 1130, No. 3, 06.04.1992, p. 317-325.

Research output: Contribution to journalArticle

@article{060b3884538c47e28dd4d9fc711b2f27,
title = "α-Fetoprotein expression in fetal kidney cells does not require enhancers",
abstract = "Expression of the α-fetoprotein (AFP) and albumin genes was examined in fetal mouse kidney by analysis of tissue mRNA pool sizes during development and transient expression assays in primary kidney tissue culture cells. AFP is expressed at a much lower level in kidney than in liver but transcription of the gene is activated early during development and repressed after birth with a time-course similar to liver. However, albumin mRNA was not detected in fetal or new born mouse kidney. Transient expression assays using AFP- and albumin-CAT (chloramphenicol acetyl transferase) vectors were employed to characterize cis-acting elements active in the regulation of AFP expression in kidney. Primary fetal liver and kidney cells in culture were used for these assays. The AFP promoter is active in kidney cells and the information necessary for tissue specific expression and developmental repression are contained within the first 1.0 kb of 5′ flanking sequences of the AFP gene. In addition, the AFP upstream enhancer elements are inactive in primary kidney cells. The mouse albumin promoter is shown to be inactive in kidney cells. The results obtained using transient expression assays are consistent with the observed low level of AFP expression, developmental repression of AFP, and the absence of expression of albumin in the mouse kidney.",
keywords = "cis-acting sequence, Gene regulation, Promoter, Tissue specific gene expression, α-Fetoprotein",
author = "Rabek, {Jeffrey P.} and Hsie, {Dora Y.} and John Papaconstantinou",
year = "1992",
month = "4",
day = "6",
doi = "10.1016/0167-4781(92)90445-6",
language = "English (US)",
volume = "1130",
pages = "317--325",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - α-Fetoprotein expression in fetal kidney cells does not require enhancers

AU - Rabek, Jeffrey P.

AU - Hsie, Dora Y.

AU - Papaconstantinou, John

PY - 1992/4/6

Y1 - 1992/4/6

N2 - Expression of the α-fetoprotein (AFP) and albumin genes was examined in fetal mouse kidney by analysis of tissue mRNA pool sizes during development and transient expression assays in primary kidney tissue culture cells. AFP is expressed at a much lower level in kidney than in liver but transcription of the gene is activated early during development and repressed after birth with a time-course similar to liver. However, albumin mRNA was not detected in fetal or new born mouse kidney. Transient expression assays using AFP- and albumin-CAT (chloramphenicol acetyl transferase) vectors were employed to characterize cis-acting elements active in the regulation of AFP expression in kidney. Primary fetal liver and kidney cells in culture were used for these assays. The AFP promoter is active in kidney cells and the information necessary for tissue specific expression and developmental repression are contained within the first 1.0 kb of 5′ flanking sequences of the AFP gene. In addition, the AFP upstream enhancer elements are inactive in primary kidney cells. The mouse albumin promoter is shown to be inactive in kidney cells. The results obtained using transient expression assays are consistent with the observed low level of AFP expression, developmental repression of AFP, and the absence of expression of albumin in the mouse kidney.

AB - Expression of the α-fetoprotein (AFP) and albumin genes was examined in fetal mouse kidney by analysis of tissue mRNA pool sizes during development and transient expression assays in primary kidney tissue culture cells. AFP is expressed at a much lower level in kidney than in liver but transcription of the gene is activated early during development and repressed after birth with a time-course similar to liver. However, albumin mRNA was not detected in fetal or new born mouse kidney. Transient expression assays using AFP- and albumin-CAT (chloramphenicol acetyl transferase) vectors were employed to characterize cis-acting elements active in the regulation of AFP expression in kidney. Primary fetal liver and kidney cells in culture were used for these assays. The AFP promoter is active in kidney cells and the information necessary for tissue specific expression and developmental repression are contained within the first 1.0 kb of 5′ flanking sequences of the AFP gene. In addition, the AFP upstream enhancer elements are inactive in primary kidney cells. The mouse albumin promoter is shown to be inactive in kidney cells. The results obtained using transient expression assays are consistent with the observed low level of AFP expression, developmental repression of AFP, and the absence of expression of albumin in the mouse kidney.

KW - cis-acting sequence

KW - Gene regulation

KW - Promoter

KW - Tissue specific gene expression

KW - α-Fetoprotein

UR - http://www.scopus.com/inward/record.url?scp=0026602803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026602803&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(92)90445-6

DO - 10.1016/0167-4781(92)90445-6

M3 - Article

C2 - 1373328

AN - SCOPUS:0026602803

VL - 1130

SP - 317

EP - 325

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 3

ER -