α1-Acid glycoprotein production in rat dorsal air pouch in response to inflammatory stimuli, dexamethasone and honey bee venom

K. Vasileiadou, G. Pantazidis, K. Papadopoulou, C. Ligoudistianou, A. Kourelis, S. Petrakis, E. Masmanidou, T. Testa, A. P. Kourounakis, L. Hadjipetrou, John Papaconstantinou, M. Yiangou

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study shows the rapid and differential production of the 40-43 kDa and the 70-90 kDa α1-acid glycoprotein (AGP) fucosylated glycoforms after treatment of the dorsal air pouch with bacterial lipopolysaccharide (LPS), HgCl2 or Freund's complete adjuvant (FCA). The 40-43 kDa and the 70-90 kDa AGP production is peaked 1-3 h post-LPS treatment. We observed that the responses to LPS and FCA are similar in that both AGP isoforms are induced whereas they differ in that the FCA exhibits a 6 h lag period. The response to HgCl2, however, exhibits the specific biphasic induction only of the 40-43 kDa AGP. The serum 40-43 kDa AGP glycoform gradually increases in response to all of the above stimulants and peaks by 24 h post- treatment. The increase of the 70-90 kDa AGP levels in the air pouch occurs in association with the accumulation of polymorphonuclear (PMN) cells while dexamethasone (DEX) increases only the 40-43 kDa AGP production in the absence of PMN accumulation. Macrophage-monocyte lineage cells forming the air pouch lining tissue may potentially be the cells that secrete the 40-43 kDa AGP while polymorphonuclear cells that infiltrate the air pouch secrete the 70-90 kDa AGP. The 40-43 kDa and 70-90 kDa AGP production induced by LPS in the air pouch precedes that of interleukin-1 (IL-1) or interleukin-6 (IL-6) while the 40-43 kDa AGP glycoform potentially increases IL-6 production by air pouch PMN exudate cells. These significant differences suggest a local pro-inflammatory role of AGP. Honeybee venom suppressed arthritis development and exhibited differential local or systemic regulation of AGP in serum vs. air pouch exudate or synovial fluid. This study with the air pouch model of facsimile synovium tissue suggests that local α1-acid glycoprotein (AGP) production may contribute to pro-inflammatory and anti-inflammatory activities during the local acute phase response or during chronic inflammatory stress as in arthritis.

Original languageEnglish (US)
Pages (from-to)63-71
Number of pages9
JournalExperimental and Molecular Pathology
Volume89
Issue number1
DOIs
StatePublished - Aug 2010

Fingerprint

Bee Venoms
Honey
Dexamethasone
Rats
Glycoproteins
Air
Acids
Lipopolysaccharides
Freund's Adjuvant
Mercuric Chloride
Exudates and Transudates
Arthritis
Interleukin-6
Tissue
Facsimile
Acute-Phase Reaction
Synovial Membrane
Macrophages
Synovial Fluid
Venoms

Keywords

  • α1-Acid glycoprotein
  • Adjuvant arthritis
  • Air pouch
  • Cytokines
  • Inflammation
  • Synovial fluid

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Vasileiadou, K., Pantazidis, G., Papadopoulou, K., Ligoudistianou, C., Kourelis, A., Petrakis, S., ... Yiangou, M. (2010). α1-Acid glycoprotein production in rat dorsal air pouch in response to inflammatory stimuli, dexamethasone and honey bee venom. Experimental and Molecular Pathology, 89(1), 63-71. https://doi.org/10.1016/j.yexmp.2010.03.008

α1-Acid glycoprotein production in rat dorsal air pouch in response to inflammatory stimuli, dexamethasone and honey bee venom. / Vasileiadou, K.; Pantazidis, G.; Papadopoulou, K.; Ligoudistianou, C.; Kourelis, A.; Petrakis, S.; Masmanidou, E.; Testa, T.; Kourounakis, A. P.; Hadjipetrou, L.; Papaconstantinou, John; Yiangou, M.

In: Experimental and Molecular Pathology, Vol. 89, No. 1, 08.2010, p. 63-71.

Research output: Contribution to journalArticle

Vasileiadou, K, Pantazidis, G, Papadopoulou, K, Ligoudistianou, C, Kourelis, A, Petrakis, S, Masmanidou, E, Testa, T, Kourounakis, AP, Hadjipetrou, L, Papaconstantinou, J & Yiangou, M 2010, 'α1-Acid glycoprotein production in rat dorsal air pouch in response to inflammatory stimuli, dexamethasone and honey bee venom', Experimental and Molecular Pathology, vol. 89, no. 1, pp. 63-71. https://doi.org/10.1016/j.yexmp.2010.03.008
Vasileiadou, K. ; Pantazidis, G. ; Papadopoulou, K. ; Ligoudistianou, C. ; Kourelis, A. ; Petrakis, S. ; Masmanidou, E. ; Testa, T. ; Kourounakis, A. P. ; Hadjipetrou, L. ; Papaconstantinou, John ; Yiangou, M. / α1-Acid glycoprotein production in rat dorsal air pouch in response to inflammatory stimuli, dexamethasone and honey bee venom. In: Experimental and Molecular Pathology. 2010 ; Vol. 89, No. 1. pp. 63-71.
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