γδ T cells promote the maturation of dendritic cells during West Nile virus infection

Hao Fang, Thomas Welte, Xin Zheng, Gwong Jen J Chang, Michael R. Holbrook, Lynn Soong, Tian Wang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

γδ T cells are important for the early control of West Nile virus (WNV) dissemination. Here, we investigated the role of γδ T cells in the regulation of CD4+ T-cell response following a WNV challenge. Splenic dendritic cells (DCs) of WNV-infected γδ T-cell-deficient (TCRδ-/-) mice displayed lower levels of CD40, CD80, CD86 and major histocompatibility complex (MHC) class II expression and interleukin-12 (IL-12) production than those of wild-type mice. Naïve DCs cocultured with WNV-infected γδ T cells showed enhanced levels of costimulatory molecules, MHC class II expression and IL-12 production. Further, coculture of CD4+ T cells from OT II transgenic mice with DCs of WNV-infected TCRδ-/- mice induced less interferon-γ (IFN-γ) and IL-2 production than with those of wild-type controls. Viral antigens were detected in WNV-infected γδ T cells.WNV infection or toll-like receptor (TLR) agonist treatment of γδ T cells induced the production of IFN-γ, tumor necrosis factor-α and IL-6, which are known to promote DC maturation. Nevertheless, the levels of TLRs 2, 3, 4 and 7 expression of WNV-infected γδ T cells were not different from those of noninfected cells. Overall, these data suggest that WNV-induced γδ T-cell activation promotes DC maturation and initiates CD4 + T-cell priming.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume59
Issue number1
DOIs
StatePublished - Jun 2010

Fingerprint

West Nile virus
Virus Diseases
Dendritic Cells
T-Lymphocytes
Interleukin-12
Major Histocompatibility Complex
Interferons
Viral Antigens
Toll-Like Receptors
Coculture Techniques
Transgenic Mice
Interleukin-2
Interleukin-6
Tumor Necrosis Factor-alpha

Keywords

  • γδ T cell
  • Dendritic cell
  • West Nile virus

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology

Cite this

γδ T cells promote the maturation of dendritic cells during West Nile virus infection. / Fang, Hao; Welte, Thomas; Zheng, Xin; Chang, Gwong Jen J; Holbrook, Michael R.; Soong, Lynn; Wang, Tian.

In: FEMS Immunology and Medical Microbiology, Vol. 59, No. 1, 06.2010, p. 71-80.

Research output: Contribution to journalArticle

Fang, Hao ; Welte, Thomas ; Zheng, Xin ; Chang, Gwong Jen J ; Holbrook, Michael R. ; Soong, Lynn ; Wang, Tian. / γδ T cells promote the maturation of dendritic cells during West Nile virus infection. In: FEMS Immunology and Medical Microbiology. 2010 ; Vol. 59, No. 1. pp. 71-80.
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