γδ T cells promote the maturation of dendritic cells during West Nile virus infection

Hao Fang, Thomas Welte, Xin Zheng, Gwong Jen J. Chang, Michael R. Holbrook, Lynn Soong, Tian Wang

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

γδ T cells are important for the early control of West Nile virus (WNV) dissemination. Here, we investigated the role of γδ T cells in the regulation of CD4+ T-cell response following a WNV challenge. Splenic dendritic cells (DCs) of WNV-infected γδ T-cell-deficient (TCRδ-/-) mice displayed lower levels of CD40, CD80, CD86 and major histocompatibility complex (MHC) class II expression and interleukin-12 (IL-12) production than those of wild-type mice. Naïve DCs cocultured with WNV-infected γδ T cells showed enhanced levels of costimulatory molecules, MHC class II expression and IL-12 production. Further, coculture of CD4+ T cells from OT II transgenic mice with DCs of WNV-infected TCRδ-/- mice induced less interferon-γ (IFN-γ) and IL-2 production than with those of wild-type controls. Viral antigens were detected in WNV-infected γδ T cells.WNV infection or toll-like receptor (TLR) agonist treatment of γδ T cells induced the production of IFN-γ, tumor necrosis factor-α and IL-6, which are known to promote DC maturation. Nevertheless, the levels of TLRs 2, 3, 4 and 7 expression of WNV-infected γδ T cells were not different from those of noninfected cells. Overall, these data suggest that WNV-induced γδ T-cell activation promotes DC maturation and initiates CD4 + T-cell priming.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume59
Issue number1
DOIs
StatePublished - Jun 2010

Keywords

  • Dendritic cell
  • West Nile virus
  • γδ T cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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