γ-Tocopherol nebulization by a lipid aerosolization device improves pulmonary function in sheep with burn and smoke inhalation injury

Atsumori Hamahata, Perenlei Enkhbaatar, Edward R. Kraft, Matthias Lange, Scott W. Leonard, Maret G. Traber, Robert A. Cox, Frank C. Schmalstieg, Hal K. Hawkins, Elbert B. Whorton, Eszter M. Horvath, Csaba Szabo, Lillian D. Traber, David Herndon, Daniel L. Traber

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Fire accident victims who sustain both thermal injury to skin and smoke inhalation have gross evidence of systemic and pulmonary oxidant damage and acute lung injury. We hypothesized that γ-tocopherol (gT), a reactive O2 and N2 scavenger, when delivered into the airway, would attenuate lung injury induced by burn and smoke inhalation. Acute lung injury was induced in chronically prepared, anesthetized sheep by 40% total burn surface area, third-degree skin burn and smoke insufflation (48 breaths of cotton smoke, < 40°C). The study groups were: (1) Sham (not injured, flaxseed oil (FO)-nebulized, n = 6); (2) SA-neb (injured, saline-nebulized, n = 6); (3) FO-neb (injured, FO-nebulized, n = 6); and (4) gT+FO-neb (injured, gT and FO-nebulized, n = 6). Nebulization was started 1 h postinjury, and 24 ml of FO with or without gT (51 mg/ml) was delivered into airways over 47 h using our newly developed lipid aerosolization device (droplet size: 2.5-5 μm). The burn- and smoke inhalation-induced pathological changes seen in the saline group were attenuated by FO nebulization; gT addition further improved pulmonary function. Pulmonary gT delivery along with a FO source may be a novel effective treatment strategy in management of patients with acute lung injury.

Original languageEnglish (US)
Pages (from-to)425-433
Number of pages9
JournalFree Radical Biology and Medicine
Volume45
Issue number4
DOIs
StatePublished - Aug 15 2008

Fingerprint

Smoke Inhalation Injury
Linseed Oil
Tocopherols
Smoke
Sheep
Lipids
Equipment and Supplies
Lung
Acute Lung Injury
Inhalation Burns
Skin
Insufflation
Lung Injury
Oxidants
Inhalation
Cotton
Accidents
Fires
Hot Temperature

Keywords

  • γ-Tocopherol
  • Acute lung injury
  • Burn
  • Free radicals
  • Ovine model
  • Smoke inhalation injury

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

γ-Tocopherol nebulization by a lipid aerosolization device improves pulmonary function in sheep with burn and smoke inhalation injury. / Hamahata, Atsumori; Enkhbaatar, Perenlei; Kraft, Edward R.; Lange, Matthias; Leonard, Scott W.; Traber, Maret G.; Cox, Robert A.; Schmalstieg, Frank C.; Hawkins, Hal K.; Whorton, Elbert B.; Horvath, Eszter M.; Szabo, Csaba; Traber, Lillian D.; Herndon, David; Traber, Daniel L.

In: Free Radical Biology and Medicine, Vol. 45, No. 4, 15.08.2008, p. 425-433.

Research output: Contribution to journalArticle

Hamahata, A, Enkhbaatar, P, Kraft, ER, Lange, M, Leonard, SW, Traber, MG, Cox, RA, Schmalstieg, FC, Hawkins, HK, Whorton, EB, Horvath, EM, Szabo, C, Traber, LD, Herndon, D & Traber, DL 2008, 'γ-Tocopherol nebulization by a lipid aerosolization device improves pulmonary function in sheep with burn and smoke inhalation injury', Free Radical Biology and Medicine, vol. 45, no. 4, pp. 425-433. https://doi.org/10.1016/j.freeradbiomed.2008.04.037
Hamahata, Atsumori ; Enkhbaatar, Perenlei ; Kraft, Edward R. ; Lange, Matthias ; Leonard, Scott W. ; Traber, Maret G. ; Cox, Robert A. ; Schmalstieg, Frank C. ; Hawkins, Hal K. ; Whorton, Elbert B. ; Horvath, Eszter M. ; Szabo, Csaba ; Traber, Lillian D. ; Herndon, David ; Traber, Daniel L. / γ-Tocopherol nebulization by a lipid aerosolization device improves pulmonary function in sheep with burn and smoke inhalation injury. In: Free Radical Biology and Medicine. 2008 ; Vol. 45, No. 4. pp. 425-433.
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N2 - Fire accident victims who sustain both thermal injury to skin and smoke inhalation have gross evidence of systemic and pulmonary oxidant damage and acute lung injury. We hypothesized that γ-tocopherol (gT), a reactive O2 and N2 scavenger, when delivered into the airway, would attenuate lung injury induced by burn and smoke inhalation. Acute lung injury was induced in chronically prepared, anesthetized sheep by 40% total burn surface area, third-degree skin burn and smoke insufflation (48 breaths of cotton smoke, < 40°C). The study groups were: (1) Sham (not injured, flaxseed oil (FO)-nebulized, n = 6); (2) SA-neb (injured, saline-nebulized, n = 6); (3) FO-neb (injured, FO-nebulized, n = 6); and (4) gT+FO-neb (injured, gT and FO-nebulized, n = 6). Nebulization was started 1 h postinjury, and 24 ml of FO with or without gT (51 mg/ml) was delivered into airways over 47 h using our newly developed lipid aerosolization device (droplet size: 2.5-5 μm). The burn- and smoke inhalation-induced pathological changes seen in the saline group were attenuated by FO nebulization; gT addition further improved pulmonary function. Pulmonary gT delivery along with a FO source may be a novel effective treatment strategy in management of patients with acute lung injury.

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