γ-tocopherol nebulization decreases oxidative stress, arginase activity, and collagen deposition after burn and smoke inhalation in the ovine model

Yusuke Yamamoto, Linda Sousse, Perenlei Enkhbaatar, Edward R. Kraft, Donald J. Deyo, Charlotte L. Wright, Alan Taylor, Maret G. Traber, Robert A. Cox, Hal K. Hawkins, Sebastian W. Rehberg, Lillian D. Traber, David Herndon, Daniel L. Traber

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

More than 20,000 burn injury victims suffer from smoke inhalation injury in the United States annually. In an ovine model of acute lung injury, γ-tocopherol had a beneficial effect when nebulized into the airway. We hypothesize that γ-tocopherol scavenges reactive oxygen species (ROS) and reactive nitrogen species resulting from burn and smoke inhalation injury and that these ROS/reactive nitrogen species activate the arginase pathway, leading to increased collagen deposition and decreased pulmonary function. To test this hypothesis, ewes were operatively prepared for chronic study, then they were randomly divided into groups (n = 8): uninjured, injured, or injured with nebulization (γ-tocopherol [950 mg/g] and α-tocopherol [40 mg/g] from hours 3 to 48 after the injury). The injury, under deep anesthesia, consisted of a 20% total body surface burn and 36 breaths of cotton smoke; all animals were killed after 3 weeks. Treatment increased lung γ-tocopherol at 3 weeks after γ-tocopherol nebulization compared with injured sheep (1.75 ± 0.62 nmol/g vs. 0.45 ± 0.06, P < 0.05). The expression of dimethylarginine dimethylaminohydrolase-2, which degrades asymmetrical dimethylarginine, a nitric oxide synthase inhibitor, significantly increases with γ-tocopherol treatment compared with injured sheep (P < 0.05). Arginase activity (0.15 ± 0.02 μM urea/μg protein vs. 0.24 ± 0.009, P < 0.05), ornithine aminotransferase (11,720 ± 888 vs. 13,170 ± 1,775), and collagen deposition (0.62 ± 0.12 μM hydroxyproline/μg protein vs. 1.02 ± 0.13, P < 0.05) significantly decrease with γ-tocopherol compared with injured animals without γ-tocopherol. The decreases in arginase and collagen with γ-tocopherol are associated with significantly increased diffusion capacity (P < 0.05) and decreased lung wet-to-dry ratio (P < 0.05). Smoke-induced chronic pulmonary dysfunction is mediated through the ROS/asymmetrical dimethylarginine/arginase pathway, and ROS scavengers such as γ-tocopherol may be a potential therapeutic management of burn patients with inhalation injury.

Original languageEnglish (US)
Pages (from-to)671-676
Number of pages6
JournalShock
Volume38
Issue number6
DOIs
StatePublished - Dec 2012

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Inhalation Burns
Arginase
Tocopherols
Smoke
Sheep
Oxidative Stress
Collagen
Reactive Oxygen Species
Smoke Inhalation Injury
Reactive Nitrogen Species
Wounds and Injuries
Lung
Ornithine-Oxo-Acid Transaminase
Acute Lung Injury
Hydroxyproline
Pulmonary Edema
Nitric Oxide Synthase
Inhalation

Keywords

  • arginine metabolism
  • Fibrosis
  • wound healing

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

γ-tocopherol nebulization decreases oxidative stress, arginase activity, and collagen deposition after burn and smoke inhalation in the ovine model. / Yamamoto, Yusuke; Sousse, Linda; Enkhbaatar, Perenlei; Kraft, Edward R.; Deyo, Donald J.; Wright, Charlotte L.; Taylor, Alan; Traber, Maret G.; Cox, Robert A.; Hawkins, Hal K.; Rehberg, Sebastian W.; Traber, Lillian D.; Herndon, David; Traber, Daniel L.

In: Shock, Vol. 38, No. 6, 12.2012, p. 671-676.

Research output: Contribution to journalArticle

Yamamoto, Y, Sousse, L, Enkhbaatar, P, Kraft, ER, Deyo, DJ, Wright, CL, Taylor, A, Traber, MG, Cox, RA, Hawkins, HK, Rehberg, SW, Traber, LD, Herndon, D & Traber, DL 2012, 'γ-tocopherol nebulization decreases oxidative stress, arginase activity, and collagen deposition after burn and smoke inhalation in the ovine model', Shock, vol. 38, no. 6, pp. 671-676. https://doi.org/10.1097/SHK.0b013e3182758759
Yamamoto, Yusuke ; Sousse, Linda ; Enkhbaatar, Perenlei ; Kraft, Edward R. ; Deyo, Donald J. ; Wright, Charlotte L. ; Taylor, Alan ; Traber, Maret G. ; Cox, Robert A. ; Hawkins, Hal K. ; Rehberg, Sebastian W. ; Traber, Lillian D. ; Herndon, David ; Traber, Daniel L. / γ-tocopherol nebulization decreases oxidative stress, arginase activity, and collagen deposition after burn and smoke inhalation in the ovine model. In: Shock. 2012 ; Vol. 38, No. 6. pp. 671-676.
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AU - Deyo, Donald J.

AU - Wright, Charlotte L.

AU - Taylor, Alan

AU - Traber, Maret G.

AU - Cox, Robert A.

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N2 - More than 20,000 burn injury victims suffer from smoke inhalation injury in the United States annually. In an ovine model of acute lung injury, γ-tocopherol had a beneficial effect when nebulized into the airway. We hypothesize that γ-tocopherol scavenges reactive oxygen species (ROS) and reactive nitrogen species resulting from burn and smoke inhalation injury and that these ROS/reactive nitrogen species activate the arginase pathway, leading to increased collagen deposition and decreased pulmonary function. To test this hypothesis, ewes were operatively prepared for chronic study, then they were randomly divided into groups (n = 8): uninjured, injured, or injured with nebulization (γ-tocopherol [950 mg/g] and α-tocopherol [40 mg/g] from hours 3 to 48 after the injury). The injury, under deep anesthesia, consisted of a 20% total body surface burn and 36 breaths of cotton smoke; all animals were killed after 3 weeks. Treatment increased lung γ-tocopherol at 3 weeks after γ-tocopherol nebulization compared with injured sheep (1.75 ± 0.62 nmol/g vs. 0.45 ± 0.06, P < 0.05). The expression of dimethylarginine dimethylaminohydrolase-2, which degrades asymmetrical dimethylarginine, a nitric oxide synthase inhibitor, significantly increases with γ-tocopherol treatment compared with injured sheep (P < 0.05). Arginase activity (0.15 ± 0.02 μM urea/μg protein vs. 0.24 ± 0.009, P < 0.05), ornithine aminotransferase (11,720 ± 888 vs. 13,170 ± 1,775), and collagen deposition (0.62 ± 0.12 μM hydroxyproline/μg protein vs. 1.02 ± 0.13, P < 0.05) significantly decrease with γ-tocopherol compared with injured animals without γ-tocopherol. The decreases in arginase and collagen with γ-tocopherol are associated with significantly increased diffusion capacity (P < 0.05) and decreased lung wet-to-dry ratio (P < 0.05). Smoke-induced chronic pulmonary dysfunction is mediated through the ROS/asymmetrical dimethylarginine/arginase pathway, and ROS scavengers such as γ-tocopherol may be a potential therapeutic management of burn patients with inhalation injury.

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