δ-bag cell peptide from the egg-laying hormone precursor of Aplysia: Processing, primary structure, and biological activity

Gregg T. Nagle, Marijke De Jong-Brink, Sherry D. Painter, Marion M J Bergamin-Sassen, James E. Blankenship, Alexander Kurosky

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    The bag cells of the marine mollusk Aplysia express a gene encoding a 271-residue egg-laying hormone (ELH) precursor that is processed into at least nine peptide products. Four of the peptides have been identified in bag cell releasates and are known to act as nonsynaptic neurotransmitters in the abdominal ganglion. The isolation, primary structure, and proposed biological activity of a fifth peptide product (δ-bag cell peptide (δ-BCP)) from the ELH precursor are described. δ-BCP was established to be a 39-residue peptide: NH 2-Asp-Gln-Asp-Glu-Gly-Asn-Phe-Arg-Arg-Phe-Pro-Thr-Asn-Ala-Val-Ser- Met-Ser-Ala-Asp-Glu-Asn-Ser-Pro-Phe-Asp-Leu-Ser-Asn-Glu-Asp-Gly-Ala-Val-Tyr-Gln- Arg-Asp-Leu-COOH. This sequence corresponds to residues 81-119 of the ELH prohormone and shares sequence identity with atrial gland peptides A and B. Significantly, synthetic δ-BCP stimulated Ca2+ uptake into mitochondria of secretory cells in the albumin gland in vitro, suggesting that the peptide regulates the cellular release of perivitelline fluid by the gland. Similar results were obtained with purified peptide A and a shorter version of δ-BCP (δ-BCP-(14-33)). These results indicate that δ-BCP belongs to a family of structurally related peptides with similar pharmacological activities that center at a conserved region of sequence corresponding to δ-BCP-(14-33).

    Original languageEnglish
    Pages (from-to)22329-22335
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume265
    Issue number36
    StatePublished - Dec 25 1990

    Fingerprint

    Bioactivity
    Hormones
    Peptides
    Processing
    Ovum
    Aplysia
    Mitochondria
    Gene encoding
    Conserved Sequence
    Mollusca
    Ganglia
    Neurotransmitter Agents
    Mollusca egg-laying hormone
    Albumins
    Pharmacology
    Fluids
    Genes

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Nagle, G. T., De Jong-Brink, M., Painter, S. D., Bergamin-Sassen, M. M. J., Blankenship, J. E., & Kurosky, A. (1990). δ-bag cell peptide from the egg-laying hormone precursor of Aplysia: Processing, primary structure, and biological activity. Journal of Biological Chemistry, 265(36), 22329-22335.

    δ-bag cell peptide from the egg-laying hormone precursor of Aplysia : Processing, primary structure, and biological activity. / Nagle, Gregg T.; De Jong-Brink, Marijke; Painter, Sherry D.; Bergamin-Sassen, Marion M J; Blankenship, James E.; Kurosky, Alexander.

    In: Journal of Biological Chemistry, Vol. 265, No. 36, 25.12.1990, p. 22329-22335.

    Research output: Contribution to journalArticle

    Nagle, GT, De Jong-Brink, M, Painter, SD, Bergamin-Sassen, MMJ, Blankenship, JE & Kurosky, A 1990, 'δ-bag cell peptide from the egg-laying hormone precursor of Aplysia: Processing, primary structure, and biological activity', Journal of Biological Chemistry, vol. 265, no. 36, pp. 22329-22335.
    Nagle GT, De Jong-Brink M, Painter SD, Bergamin-Sassen MMJ, Blankenship JE, Kurosky A. δ-bag cell peptide from the egg-laying hormone precursor of Aplysia: Processing, primary structure, and biological activity. Journal of Biological Chemistry. 1990 Dec 25;265(36):22329-22335.
    Nagle, Gregg T. ; De Jong-Brink, Marijke ; Painter, Sherry D. ; Bergamin-Sassen, Marion M J ; Blankenship, James E. ; Kurosky, Alexander. / δ-bag cell peptide from the egg-laying hormone precursor of Aplysia : Processing, primary structure, and biological activity. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 36. pp. 22329-22335.
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    AU - Painter, Sherry D.

    AU - Bergamin-Sassen, Marion M J

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