14,15-dihydroxyeicosatrienoic acid (DHET) potentiates bradykinm-mduced relaxation of porcine coronary arteries

N. L. Weintraub, Xiang Fang, P. Chatterjee, D. E. Williard, M. Van Rollins, A. A. Spector

Research output: Contribution to journalArticle

Abstract

DHETs are potent coronary vasodilators formed from epoxyeicosatrienoic acids (EETs), cytochrome P450 epoxygenase metabolites of arachidonic acid (AA). DHETs can be taken up by endothelial (endo) cells and incorporated into phospholipids that participate in cell signal transduction. However, the functional significance of DHET incorporation into endo cell lipids is unknown. We examined relaxation to bradykinin (BK), a potent stimulator of endo phospholipid hydrolysis, before (control) and after (post DHET) incubation with 14,15-DHET In porcine coronary artery (PCA) rings submaximally contracted with a thromboxane mimetic, U46619, 14,15-DHET (0.1-5 μM) produced sustained relaxation (59 ± 9% [mean±SE] at 5 μM); whereas relaxation to BK (0.3-100 nM) was transient. After 30 min incubation with 14,15-DHET (5 μM), BK-induced relaxation was potentiated in both magnitude (table) and duration. Pretreatment with 2 μM triacsin C (an inhibitor of acyl-CoA synthases), which blocked [3H]AA incorporation into endo cell lipids and PCA rings, blocked 14,15-DHET-induced potentiation of relaxation to BK. We conclude that incorporation of 14,15-DHET into cell lipids results in potentiation of BK-induced relaxation, providing the first evidence that DHETs incorporated into cell lipids are capable of altering cellular function. BK concentration (nM) %relaxation to BK 1 3 10 30 100 control 11±8 39±11 55±10 65±9 77±8 post DHET 39±13*81±9*92±7*92±7 *92±7**p<0.05.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

coronary vessels
bradykinin
Bradykinin
Coronary Vessels
Swine
swine
acids
Endothelial cells
Lipids
endothelial cells
Endothelial Cells
Arachidonic Acid
lipids
arachidonic acid
Phospholipids
phospholipids
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Signal transduction
Acyl Coenzyme A
14,15-dihydroxyeicosatrienoic acid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Weintraub, N. L., Fang, X., Chatterjee, P., Williard, D. E., Van Rollins, M., & Spector, A. A. (1997). 14,15-dihydroxyeicosatrienoic acid (DHET) potentiates bradykinm-mduced relaxation of porcine coronary arteries. FASEB Journal, 11(3).

14,15-dihydroxyeicosatrienoic acid (DHET) potentiates bradykinm-mduced relaxation of porcine coronary arteries. / Weintraub, N. L.; Fang, Xiang; Chatterjee, P.; Williard, D. E.; Van Rollins, M.; Spector, A. A.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Weintraub, NL, Fang, X, Chatterjee, P, Williard, DE, Van Rollins, M & Spector, AA 1997, '14,15-dihydroxyeicosatrienoic acid (DHET) potentiates bradykinm-mduced relaxation of porcine coronary arteries', FASEB Journal, vol. 11, no. 3.
Weintraub, N. L. ; Fang, Xiang ; Chatterjee, P. ; Williard, D. E. ; Van Rollins, M. ; Spector, A. A. / 14,15-dihydroxyeicosatrienoic acid (DHET) potentiates bradykinm-mduced relaxation of porcine coronary arteries. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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AU - Spector, A. A.

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