30 nm Chromatin Fibre Decompaction Requires both H4-K16 Acetylation and Linker Histone Eviction

Philip J J Robinson, Woojin An, Andrew Routh, Fabrizio Martino, Lynda Chapman, Robert G. Roeder, Daniela Rhodes

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

The mechanism by which chromatin is decondensed to permit access to DNA is largely unknown. Here, using a model nucleosome array reconstituted from recombinant histone octamers, we have defined the relative contribution of the individual histone octamer N-terminal tails as well as the effect of a targeted histone tail acetylation on the compaction state of the 30 nm chromatin fiber. This study goes beyond previous studies as it is based on a nucleosome array that is very long (61 nucleosomes) and contains a stoichiometric concentration of bound linker histone, which is essential for the formation of the 30 nm chromatin fiber. We find that compaction is regulated in two steps: Introduction of H4 acetylated to 30% on K16 inhibits compaction to a greater degree than deletion of the H4 N-terminal tail. Further decompaction is achieved by removal of the linker histone.

Original languageEnglish (US)
Pages (from-to)816-825
Number of pages10
JournalJournal of Molecular Biology
Volume381
Issue number4
DOIs
StatePublished - Sep 12 2008
Externally publishedYes

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Acetylation
Histones
Chromatin
Nucleosomes
DNA

Keywords

  • 30 nm fibre
  • chromatin
  • histone acetylation
  • linker histone
  • MOF

ASJC Scopus subject areas

  • Virology

Cite this

30 nm Chromatin Fibre Decompaction Requires both H4-K16 Acetylation and Linker Histone Eviction. / Robinson, Philip J J; An, Woojin; Routh, Andrew; Martino, Fabrizio; Chapman, Lynda; Roeder, Robert G.; Rhodes, Daniela.

In: Journal of Molecular Biology, Vol. 381, No. 4, 12.09.2008, p. 816-825.

Research output: Contribution to journalArticle

Robinson, Philip J J ; An, Woojin ; Routh, Andrew ; Martino, Fabrizio ; Chapman, Lynda ; Roeder, Robert G. ; Rhodes, Daniela. / 30 nm Chromatin Fibre Decompaction Requires both H4-K16 Acetylation and Linker Histone Eviction. In: Journal of Molecular Biology. 2008 ; Vol. 381, No. 4. pp. 816-825.
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