3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012

Reed F. Johnson, Ulas Bagci, Lauren Keith, Xianchun Tang, Daniel J. Mollura, Larry Zeitlin, Jing Qin, Louis Huzella, Christopher J. Bartos, Natasha Bohorova, Ognian Bohorov, Charles Goodman, Do H. Kim, Michael H. Paulty, Jesus Velasco, Kevin J. Whaley, Joshua C. Johnson, James Pettitt, Britini L. Ork, Jeffrey SolomonNicholas Oberlander, Quan Zhu, Jiusong Sun, Michael R. Holbrook, Gene G. Olinger, Ralph S. Baric, Lisa E. Hensley, Peter B. Jahrling, Wayne A. Marasco

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) was identified in 2012 as the causative agent of a severe, lethal respiratory disease occurring across several countries in the Middle East. To date there have been over 1600 laboratory confirmed cases of MERS-CoV in 26 countries with a case fatality rate of 36%. Given the endemic region, it is possible that MERS-CoV could spread during the annual Hajj pilgrimage, necessitating countermeasure development. In this report, we describe the clinical and radiographic changes of rhesus monkeys following infection with 5×106 PFU MERS-CoV Jordan-n3/2012. Two groups of NHPs were treated with either a human anti-MERS monoclonal antibody 3B11-N or E410-N, an anti-HIV antibody. MERS-CoV Jordan-n3/2012 infection resulted in quantifiable changes by computed tomography, but limited other clinical signs of disease. 3B11-N treated subjects developed significantly reduced lung pathology when compared to infected, untreated subjects, indicating that this antibody may be a suitable MERS-CoV treatment.

Original languageEnglish (US)
Pages (from-to)49-58
Number of pages10
JournalVirology
Volume490
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

Keywords

  • Animal model, MERS
  • Antibody therapy
  • Human monoclonal antibody therapy
  • MERS-CoV
  • Respiratory syndrome

ASJC Scopus subject areas

  • Virology

Fingerprint

Dive into the research topics of '3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012'. Together they form a unique fingerprint.

Cite this