4-hydroxynonenal interaction with rhodopsin

Frederik J.G.M. Van Kuijk

doi:10.1006/bbrc.1996.5942

4-hydroxynonenal interaction with rhodopsin

Frederik J.G.M. Van Kuijk

Ophthalmology & Visual Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

4-Hydroxynonenal binds easily to rhodopsin and this was accompanied by a decrease in measurable sulfhydryl groups. Analysis of tryptic digests of the rhodopsin-HNE adduct by high performance liquid chromatography revealed that several peptides present in the digests of rhodopsin disappeared, whereas HNE modified peptides not originally present were found in digests of the rhodopsin-HNE adduct. Matrix assisted laser desorption time of flight mass spectrometry showed that up to ten molecules of HNE bound to rhodopsin.

Original language	English (US)
Pages (from-to)	275-279
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	230
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1996.5942
State	Published - Jan 13 1997

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "4-Hydroxynonenal binds easily to rhodopsin and this was accompanied by a decrease in measurable sulfhydryl groups. Analysis of tryptic digests of the rhodopsin-HNE adduct by high performance liquid chromatography revealed that several peptides present in the digests of rhodopsin disappeared, whereas HNE modified peptides not originally present were found in digests of the rhodopsin-HNE adduct. Matrix assisted laser desorption time of flight mass spectrometry showed that up to ten molecules of HNE bound to rhodopsin.",

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AB - 4-Hydroxynonenal binds easily to rhodopsin and this was accompanied by a decrease in measurable sulfhydryl groups. Analysis of tryptic digests of the rhodopsin-HNE adduct by high performance liquid chromatography revealed that several peptides present in the digests of rhodopsin disappeared, whereas HNE modified peptides not originally present were found in digests of the rhodopsin-HNE adduct. Matrix assisted laser desorption time of flight mass spectrometry showed that up to ten molecules of HNE bound to rhodopsin.

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4-hydroxynonenal interaction with rhodopsin

Abstract

ASJC Scopus subject areas

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