4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus

Rui Zhang, Corey F. Hryc, Yao Cong, Xiangan Liu, Joanita Jakana, Rodion Gorchakov, Matthew L. Baker, Scott Weaver, Wah Chiu

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Venezuelan equine encephalitis virus (VEEV), a member of the membrane-containing Alphavirus genus, is a human and equine pathogen, and has been developed as a biological weapon. Using electron cryo-microscopy (cryo-EM), we determined the structure of an attenuated vaccine strain, TC-83, of VEEV to 4.4 Å resolution. Our density map clearly resolves regions (including E1, E2 transmembrane helices and cytoplasmic tails) that were missing in the crystal structures of domains of alphavirus subunits. These new features are implicated in the fusion, assembly and budding processes of alphaviruses. Furthermore, our map reveals the unexpected E3 protein, which is cleaved and generally thought to be absent in the mature VEEV. Our structural results suggest a mechanism for the initial stage of nucleocapsid core formation, and shed light on the virulence attenuation, host recognition and neutralizing activities of VEEV and other alphavirus pathogens.

Original languageEnglish (US)
Pages (from-to)3854-3863
Number of pages10
JournalEMBO Journal
Volume30
Issue number18
DOIs
StatePublished - Sep 2011

Fingerprint

Venezuelan Equine Encephalitis Viruses
Alphavirus
Viruses
Pathogens
Biological Warfare Agents
Cryoelectron Microscopy
Nucleocapsid
Attenuated Vaccines
Horses
Virulence
Tail
Microscopic examination
Fusion reactions
Crystal structure
Membranes
Electrons
Proteins

Keywords

  • alphavirus
  • bioweapon
  • cryo-EM
  • modelling
  • VEEV

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Zhang, R., Hryc, C. F., Cong, Y., Liu, X., Jakana, J., Gorchakov, R., ... Chiu, W. (2011). 4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus. EMBO Journal, 30(18), 3854-3863. https://doi.org/10.1038/emboj.2011.261

4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus. / Zhang, Rui; Hryc, Corey F.; Cong, Yao; Liu, Xiangan; Jakana, Joanita; Gorchakov, Rodion; Baker, Matthew L.; Weaver, Scott; Chiu, Wah.

In: EMBO Journal, Vol. 30, No. 18, 09.2011, p. 3854-3863.

Research output: Contribution to journalArticle

Zhang, R, Hryc, CF, Cong, Y, Liu, X, Jakana, J, Gorchakov, R, Baker, ML, Weaver, S & Chiu, W 2011, '4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus', EMBO Journal, vol. 30, no. 18, pp. 3854-3863. https://doi.org/10.1038/emboj.2011.261
Zhang R, Hryc CF, Cong Y, Liu X, Jakana J, Gorchakov R et al. 4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus. EMBO Journal. 2011 Sep;30(18):3854-3863. https://doi.org/10.1038/emboj.2011.261
Zhang, Rui ; Hryc, Corey F. ; Cong, Yao ; Liu, Xiangan ; Jakana, Joanita ; Gorchakov, Rodion ; Baker, Matthew L. ; Weaver, Scott ; Chiu, Wah. / 4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus. In: EMBO Journal. 2011 ; Vol. 30, No. 18. pp. 3854-3863.
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