5-ALA induced PpIX fluorescence spectroscopy in neurosurgery: a review

A. Gautheron, J. D. Bernstock, T. Picart, J. Guyotat, P. A. Valdés, B. Montcel

Research output: Contribution to journalReview articlepeer-review


The review begins with an overview of the fundamental principles/physics underlying light, fluorescence, and other light-matter interactions in biological tissues. It then focuses on 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) fluorescence spectroscopy methods used in neurosurgery (e.g., intensity, time-resolved) and in so doing, describe their specific features (e.g., hardware requirements, main processing methods) as well as their strengths and limitations. Finally, we review current clinical applications and future directions of 5-ALA-induced protoporphyrin IX (PpIX) fluorescence spectroscopy in neurosurgery.

Original languageEnglish (US)
Article number1310282
JournalFrontiers in Neuroscience
StatePublished - 2024


  • 5-aminolevulinic acid (5-ALA)
  • brain tumors
  • fluorescence guided surgery
  • fluorescence spectroscopy
  • image guidance
  • neurooncology
  • neurosurgery
  • protoporphyrin IX (PpIX)

ASJC Scopus subject areas

  • General Neuroscience


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