5-HT1B and 5-HT2 serotonin binding sites in cultured Wistar-Kyoto rat aortic smooth muscle cells

Allahyar Jazayeri, Walter Meyer, Thomas A. Kent

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We studied [3H]serotonin ([3H]5-HT) binding on cultured arterial smooth muscle cells from rat aorta. We found a high and low affinity binding site. Binding to the higher affinity site could be displaced by drugs in an order corresponding most closely with the 5-HT1B subtype, and high affinity [125I]iodo-cyanopindolol binding was also found. We found evidence for a 5-HT2 subtype using [3H]ketanserin binding, with similar results whether specific binding was determined using unlabelled ketanserin, methysergide or mianserin.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalEuropean Journal of Pharmacology
Volume169
Issue number1
DOIs
StatePublished - Oct 4 1989

Fingerprint

Ketanserin
Inbred WKY Rats
Smooth Muscle Myocytes
Serotonin
Binding Sites
Mianserin
Methysergide
Aorta
Pharmaceutical Preparations
cyanopindolol

Keywords

  • (Tissue culture)
  • 5-HT binding sites
  • 5-HT binding sites
  • 5-Hydroxytryptamine (5-HT, serotonin)
  • Smooth muscle cells (vascular)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

5-HT1B and 5-HT2 serotonin binding sites in cultured Wistar-Kyoto rat aortic smooth muscle cells. / Jazayeri, Allahyar; Meyer, Walter; Kent, Thomas A.

In: European Journal of Pharmacology, Vol. 169, No. 1, 04.10.1989, p. 183-187.

Research output: Contribution to journalArticle

Jazayeri, Allahyar ; Meyer, Walter ; Kent, Thomas A. / 5-HT1B and 5-HT2 serotonin binding sites in cultured Wistar-Kyoto rat aortic smooth muscle cells. In: European Journal of Pharmacology. 1989 ; Vol. 169, No. 1. pp. 183-187.
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