5-HT1B and 5-HT2 serotonin binding sites in cultured Wistar-Kyoto rat aortic smooth muscle cells

Allahyar Jazayeri, Walter J. Meyer, Thomas A. Kent

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We studied [3H]serotonin ([3H]5-HT) binding on cultured arterial smooth muscle cells from rat aorta. We found a high and low affinity binding site. Binding to the higher affinity site could be displaced by drugs in an order corresponding most closely with the 5-HT1B subtype, and high affinity [125I]iodo-cyanopindolol binding was also found. We found evidence for a 5-HT2 subtype using [3H]ketanserin binding, with similar results whether specific binding was determined using unlabelled ketanserin, methysergide or mianserin.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalEuropean Journal of Pharmacology
Volume169
Issue number1
DOIs
StatePublished - Oct 4 1989

Keywords

  • (Tissue culture)
  • 5-HT binding sites
  • 5-HT binding sites
  • 5-Hydroxytryptamine (5-HT, serotonin)
  • Smooth muscle cells (vascular)

ASJC Scopus subject areas

  • Pharmacology

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