8-Oxoguanine DNA glycosylase-1 links DNA repair to cellular signaling via the activation of the small GTPase Rac1

Gyorgy Hajas, Attila Bacsi, Leopoldo Aguilera-Aguirre, Muralidhar L. Hegde, K. Hazra Tapas, Sanjiv Sur, Zsolt Radak, Xueqing Ba, Istvan Boldogh

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

8-Oxo-7,8-dihydroguanine (8-oxoG) is one of the most abundant DNA base lesions induced by reactive oxygen species (ROS). Accumulation of 8-oxoG in the mammalian genome is considered a marker of oxidative stress, to be causally linked to inflammation, and is thought to contribute to aging processes and various aging-related diseases. Unexpectedly, mice that lack 8-oxoguanine DNA glycosylase-1 (OGG1) activity and accumulate 8-oxoG in their genome have a normal phenotype and longevity; in fact, they show increased resistance to both inflammation and oxidative stress. OGG1 excises and generates free 8-oxoG base during DNA base-excision repair (BER) processes. In the present study, we report that in the presence of the 8-oxoG base, OGG1 physically interacts with guanine nucleotide-free and GDP-bound Rac1 protein. This interaction results in rapid GDP→GTP, but not GTP→GDP, exchange in vitro. Importantly, a rise in the intracellular 8-oxoG base levels increases the proportion of GTP-bound Rac1. In turn Rac1-GTP mediates an increase in ROS levels via nuclear membrane-associated NADPH oxidase type 4. These results show a novel mechanism by which OGG1 in complex with 8-oxoG is linked to redox signaling and cellular responses.

Original languageEnglish (US)
Pages (from-to)384-394
Number of pages11
JournalFree Radical Biology and Medicine
Volume61
DOIs
StatePublished - 2013

Fingerprint

DNA Glycosylases
Cell signaling
Monomeric GTP-Binding Proteins
DNA Repair
Repair
Chemical activation
DNA
Oxidative stress
Guanosine Triphosphate
Reactive Oxygen Species
rac1 GTP-Binding Protein
Oxidative Stress
Genome
Inflammation
Genes
Aging of materials
Guanine Nucleotides
NADPH Oxidase
Nuclear Envelope
8-hydroxyguanine

Keywords

  • 8-Oxoguanine
  • 8-Oxoguanine DNA glycosylase-1
  • Rac1 GTPase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

8-Oxoguanine DNA glycosylase-1 links DNA repair to cellular signaling via the activation of the small GTPase Rac1. / Hajas, Gyorgy; Bacsi, Attila; Aguilera-Aguirre, Leopoldo; Hegde, Muralidhar L.; Tapas, K. Hazra; Sur, Sanjiv; Radak, Zsolt; Ba, Xueqing; Boldogh, Istvan.

In: Free Radical Biology and Medicine, Vol. 61, 2013, p. 384-394.

Research output: Contribution to journalArticle

Hajas, Gyorgy ; Bacsi, Attila ; Aguilera-Aguirre, Leopoldo ; Hegde, Muralidhar L. ; Tapas, K. Hazra ; Sur, Sanjiv ; Radak, Zsolt ; Ba, Xueqing ; Boldogh, Istvan. / 8-Oxoguanine DNA glycosylase-1 links DNA repair to cellular signaling via the activation of the small GTPase Rac1. In: Free Radical Biology and Medicine. 2013 ; Vol. 61. pp. 384-394.
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