A 27-amino-acid synthetic peptide corresponding to the NH 2-terminal zinc-binding domain of endostatin is responsible for its antitumor activity

Robert M Tjin Tham Sjin, Ronit Satchi-Fainaro, Amy E. Birsner, V-M Ramanujam, Judah Folkman, Kashi Javaherian

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The first recombinant endostatin that elicited strong anti-tumor activity was expressed in Escherichia coli and administered as a suspension. Under these conditions, the protein retained its full antiangiogenic activity. Lack of requirement for a folded structure prompted us to investigate antitumor properties of synthetic peptides corresponding to different regions of endostatin. Here, we show that the entire antitumor, antimigration, and antipermeability activities of endostatin are mimicked by a 27-amino-acid peptide corresponding to the NH2-terminal domain of endostatin. This peptide contains three histidines that are responsible for zinc binding. Mutations of the zinc-binding histidines abolished its anti-tumor and antimigration activities, but not antipermeability properties.

Original languageEnglish (US)
Pages (from-to)3656-3663
Number of pages8
JournalCancer Research
Volume65
Issue number9
DOIs
StatePublished - May 1 2005

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Endostatins
Zinc
Amino Acids
Peptides
Histidine
Neoplasms
Suspensions
Escherichia coli
Mutation
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

A 27-amino-acid synthetic peptide corresponding to the NH 2-terminal zinc-binding domain of endostatin is responsible for its antitumor activity. / Sjin, Robert M Tjin Tham; Satchi-Fainaro, Ronit; Birsner, Amy E.; Ramanujam, V-M; Folkman, Judah; Javaherian, Kashi.

In: Cancer Research, Vol. 65, No. 9, 01.05.2005, p. 3656-3663.

Research output: Contribution to journalArticle

Sjin, Robert M Tjin Tham ; Satchi-Fainaro, Ronit ; Birsner, Amy E. ; Ramanujam, V-M ; Folkman, Judah ; Javaherian, Kashi. / A 27-amino-acid synthetic peptide corresponding to the NH 2-terminal zinc-binding domain of endostatin is responsible for its antitumor activity. In: Cancer Research. 2005 ; Vol. 65, No. 9. pp. 3656-3663.
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