A cell-microelectronic sensing technique for the screening of cytoprotective compounds

Béla Ózsvári, László G. Puskás, Lajos I. Nagy, Iván Kanizsai, Márió Gyuris, Ramóna Madácsi, Liliána Z. Fehér, Domokos Gerö, Csaba Szabó

    Research output: Contribution to journalArticle

    32 Scopus citations

    Abstract

    In recent years, a new cell-based high throughput paradigm has emerged, which seeks to identify novel, pharmacologically active cytoprotective compounds. The essence of this approach is to create experimental models of cell injury relevant for a particular disease by establishing in vitro cell-based models, followed by high-throughput testing of compounds that affect the cellular response in a desired manner. Prior approaches typically used simple end-point analyses. To assess the cytoprotective effects of novel drug candidates in real-time, we have applied a cell-microelectronic sensing technique (RT-CES), which measures changes in the impedance of individual microelectronic wells that correlates linearly with cell index (reflecting cell number, adherence and cell growth), thereby allowing the continuous determination of cell viability during oxidative stress. In vitro cytotoxicity was elicited by hydrogen peroxide in myocytes (H9c2) and hepatocytes (Hep3B). Cells were post-treated at 30 min with various reference molecules and novel cytoprotective compounds. Cytoprotection detected in the RT-CES system correlated well with the results of two classical end-pointbased methods (improvement in MTT and reduction of LDH release). The RT-CES method, when used as described in the current report, is suitable for the screening of molecular libraries to identify molecules or molecule combinations that attenuate oxidative stress-induced cell damage.

    Original languageEnglish (US)
    Pages (from-to)525-530
    Number of pages6
    JournalInternational journal of molecular medicine
    Volume25
    Issue number4
    DOIs
    StatePublished - Mar 12 2010

    Keywords

    • Cell electronic sensing
    • Cytoprotection
    • Oxidative stress

    ASJC Scopus subject areas

    • Genetics

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  • Cite this

    Ózsvári, B., Puskás, L. G., Nagy, L. I., Kanizsai, I., Gyuris, M., Madácsi, R., Fehér, L. Z., Gerö, D., & Szabó, C. (2010). A cell-microelectronic sensing technique for the screening of cytoprotective compounds. International journal of molecular medicine, 25(4), 525-530. https://doi.org/10.3892/ijmm-00000373