Incorporation of [3H] leucine into oligomeric α-crystallin via individual subunits has been measured in epithelial cells and cortex fiber cells from adult bovine lenses in vitro. Our data show that the ratio of [3H] leucine incorporation via subunits αB2 and αA2 is shifted from a value of about 1:2 in epithelial cells to a value of about 1:3 in fiber cells. Thus, in this system, cellular differentiation is accompanied by a change in the stoichiometry of assembly of individual subunits to form the oligomeric α-crystallin molecule. These results indicate possible changes in the rates of synthesis of individual α-crystallin subunits.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 15 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology