A change in the stoichiometry of assembly of bovine lens α-crystallin subunits in relation to cellular differentiation

Jean Delcour; John Papaconstantinou

doi:10.1016/S0006-291X(74)80367-0

A change in the stoichiometry of assembly of bovine lens α-crystallin subunits in relation to cellular differentiation

Jean Delcour
, John Papaconstantinou

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Incorporation of [³H] leucine into oligomeric α-crystallin via individual subunits has been measured in epithelial cells and cortex fiber cells from adult bovine lenses in vitro. Our data show that the ratio of [³H] leucine incorporation via subunits αB₂ and αA₂ is shifted from a value of about 1:2 in epithelial cells to a value of about 1:3 in fiber cells. Thus, in this system, cellular differentiation is accompanied by a change in the stoichiometry of assembly of individual subunits to form the oligomeric α-crystallin molecule. These results indicate possible changes in the rates of synthesis of individual α-crystallin subunits.

Original language	English (US)
Pages (from-to)	134-141
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	57
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(74)80367-0
State	Published - Mar 15 1974
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Incorporation of [3H] leucine into oligomeric α-crystallin via individual subunits has been measured in epithelial cells and cortex fiber cells from adult bovine lenses in vitro. Our data show that the ratio of [3H] leucine incorporation via subunits αB2 and αA2 is shifted from a value of about 1:2 in epithelial cells to a value of about 1:3 in fiber cells. Thus, in this system, cellular differentiation is accompanied by a change in the stoichiometry of assembly of individual subunits to form the oligomeric α-crystallin molecule. These results indicate possible changes in the rates of synthesis of individual α-crystallin subunits.",

author = "Jean Delcour and John Papaconstantinou",

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year = "1974",

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doi = "10.1016/S0006-291X(74)80367-0",

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AU - Delcour, Jean

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PY - 1974/3/15

Y1 - 1974/3/15

N2 - Incorporation of [3H] leucine into oligomeric α-crystallin via individual subunits has been measured in epithelial cells and cortex fiber cells from adult bovine lenses in vitro. Our data show that the ratio of [3H] leucine incorporation via subunits αB2 and αA2 is shifted from a value of about 1:2 in epithelial cells to a value of about 1:3 in fiber cells. Thus, in this system, cellular differentiation is accompanied by a change in the stoichiometry of assembly of individual subunits to form the oligomeric α-crystallin molecule. These results indicate possible changes in the rates of synthesis of individual α-crystallin subunits.

AB - Incorporation of [3H] leucine into oligomeric α-crystallin via individual subunits has been measured in epithelial cells and cortex fiber cells from adult bovine lenses in vitro. Our data show that the ratio of [3H] leucine incorporation via subunits αB2 and αA2 is shifted from a value of about 1:2 in epithelial cells to a value of about 1:3 in fiber cells. Thus, in this system, cellular differentiation is accompanied by a change in the stoichiometry of assembly of individual subunits to form the oligomeric α-crystallin molecule. These results indicate possible changes in the rates of synthesis of individual α-crystallin subunits.

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A change in the stoichiometry of assembly of bovine lens α-crystallin subunits in relation to cellular differentiation

Abstract

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