A chemical approach for site-specific identification of NMR signals from protein side-chain NH<inf>3</inf><sup>+</sup> groups forming intermolecular ion pairs in protein-nucleic acid complexes

Kurtis M. Anderson, Dan Nguyen, Alexandre Esadze, Levani Zandrashvili, David G. Gorenstein, Junji Iwahara

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Abstract Protein-nucleic acid interactions involve intermolecular ion pairs of protein side-chain and DNA or RNA phosphate groups. Using three protein-DNA complexes, we demonstrate that site-specific oxygen-to-sulfur substitution in phosphate groups allows for identification of NMR signals from the protein side-chain NH<inf>3</inf> <sup>+</sup> groups forming the intermolecular ion pairs. A characteristic change in their <sup>1</sup>H and <sup>15</sup>N resonances upon this modification (i.e., substitution of phosphate to phosphorodithioate) can represent a signature of an intermolecular ion pair. Hydrogen-bond scalar coupling between protein side-chain <sup>15</sup>N and DNA phosphorodithiaote <sup>31</sup>P nuclei provides direct confirmation of the intermolecular ion pair. The same approach is likely applicable to protein-RNA complexes as well.

Original languageEnglish (US)
Article number9909
Pages (from-to)1-5
Number of pages5
JournalJournal of Biomolecular NMR
Volume62
Issue number1
DOIs
StatePublished - Jun 1 2015

Fingerprint

Nucleic Acids
Nuclear magnetic resonance
Ions
Proteins
Phosphates
DNA
Substitution reactions
RNA
Social Identification
Sulfur
Hydrogen
Hydrogen bonds
Oxygen

Keywords

  • Hydrogen bonds
  • Ion pairs
  • NH<inf>3</inf><sup>+</sup> groups
  • Protein side chains
  • Protein-nucleic acid interactions

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry

Cite this

A chemical approach for site-specific identification of NMR signals from protein side-chain NH<inf>3</inf><sup>+</sup> groups forming intermolecular ion pairs in protein-nucleic acid complexes. / Anderson, Kurtis M.; Nguyen, Dan; Esadze, Alexandre; Zandrashvili, Levani; Gorenstein, David G.; Iwahara, Junji.

In: Journal of Biomolecular NMR, Vol. 62, No. 1, 9909, 01.06.2015, p. 1-5.

Research output: Contribution to journalArticle

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AU - Zandrashvili, Levani

AU - Gorenstein, David G.

AU - Iwahara, Junji

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AB - Abstract Protein-nucleic acid interactions involve intermolecular ion pairs of protein side-chain and DNA or RNA phosphate groups. Using three protein-DNA complexes, we demonstrate that site-specific oxygen-to-sulfur substitution in phosphate groups allows for identification of NMR signals from the protein side-chain NH3 + groups forming the intermolecular ion pairs. A characteristic change in their 1H and 15N resonances upon this modification (i.e., substitution of phosphate to phosphorodithioate) can represent a signature of an intermolecular ion pair. Hydrogen-bond scalar coupling between protein side-chain 15N and DNA phosphorodithiaote 31P nuclei provides direct confirmation of the intermolecular ion pair. The same approach is likely applicable to protein-RNA complexes as well.

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