A chemical-genetic approach for precise spatio-temporal control of cellular signaling

Shuyun Dong, John Allen, Martilias Farrell, Bryan L. Roth

Research output: Contribution to journalReview article

37 Citations (Scopus)

Abstract

Recently we have perfected a chemical-genetic approach to gain precise spatio-temporal control of cellular signaling. This approach entails the cell-type specific expression of mutant G-protein coupled receptors which have been evolved to be activated by the pharmacologically inert drug-like small molecule clozapine N-oxide. We have named these mutant GPCRs DREADDs (Designer Receptors Exclusively Activated by Designer Drugs). In this paper we will first review recent applications of this technology for the remote control of neuronal and non-neuronal signaling. Next, we will also introduce new variants which could be useful for the control of cellular signaling in discrete cellular compartments. Finally, we will suggest future basic science and therapeutic applications of this general technology.

Original languageEnglish (US)
Pages (from-to)1376-1380
Number of pages5
JournalMolecular BioSystems
Volume6
Issue number8
DOIs
StatePublished - Aug 2010
Externally publishedYes

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Designer Drugs
Technology
Mutant Proteins
G-Protein-Coupled Receptors
Pharmaceutical Preparations
Therapeutics
clozapine N-oxide

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

A chemical-genetic approach for precise spatio-temporal control of cellular signaling. / Dong, Shuyun; Allen, John; Farrell, Martilias; Roth, Bryan L.

In: Molecular BioSystems, Vol. 6, No. 8, 08.2010, p. 1376-1380.

Research output: Contribution to journalReview article

Dong, Shuyun ; Allen, John ; Farrell, Martilias ; Roth, Bryan L. / A chemical-genetic approach for precise spatio-temporal control of cellular signaling. In: Molecular BioSystems. 2010 ; Vol. 6, No. 8. pp. 1376-1380.
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