A chemical-genetic approach for precise spatio-temporal control of cellular signaling

Shuyun Dong, John A. Allen, Martilias Farrell, Bryan L. Roth

Research output: Contribution to journalReview article

39 Scopus citations

Abstract

Recently we have perfected a chemical-genetic approach to gain precise spatio-temporal control of cellular signaling. This approach entails the cell-type specific expression of mutant G-protein coupled receptors which have been evolved to be activated by the pharmacologically inert drug-like small molecule clozapine N-oxide. We have named these mutant GPCRs DREADDs (Designer Receptors Exclusively Activated by Designer Drugs). In this paper we will first review recent applications of this technology for the remote control of neuronal and non-neuronal signaling. Next, we will also introduce new variants which could be useful for the control of cellular signaling in discrete cellular compartments. Finally, we will suggest future basic science and therapeutic applications of this general technology.

Original languageEnglish (US)
Pages (from-to)1376-1380
Number of pages5
JournalMolecular BioSystems
Volume6
Issue number8
DOIs
StatePublished - Aug 1 2010

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ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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