A collagen-coated PGA conduit for interpositional-jump graft with end-to-side neurorrhaphy for treating facial nerve paralysis in rat

Yosuke Niimi, Hajime Matsumine, Yuichi Takeuchi, Osaki Hironobu, Satoshi Tsunoda, Mariko Miyata, Masayuki Yamato, Hiroyuki Sakurai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: This study investigated the potential of collagen-coated polyglycolic acid (PGA) tube with interpositional jump graft (IPJG) in rat. Materials and Methods: A total of 16 Lewis rats were used in this study. Facial nerve paralysis was created by ligating facial nerve trunk with a ligature clip. The rats were divided into 3 groups. Nerve conduit group (n = 6) were treated by IPJG with collagen-coated PGA tubes between the facial nerve trunks and the hypoglossal nerves. Autograft group (n = 6) were treated by IPJG with the greater auricular nerves. As the control group (n = 4), non-treated-model rats with facial nerve paralysis were used. The number of myelinated fibers, fiber diameter, axon diameter, myelin thickness, and g-ratio, were analyzed histologically at 13 weeks after surgery. Compound muscle action potential (CMAP) and retrograde tracing were measured. Result: Although the number of myelinated fibers in autograft group (1957 ± 775) had significantly higher than that of nerve conduit group (90 ± 41, P <.05), the nerve conduit group showed the regeneration of myelinated nerve axons. CMAP amplitude values of the autograft (4706 ± 1154 µV) and the nerve conduit groups (4119 ± 1397 µV) were significantly higher than that of the control group (915 ± 789 µV, P <.05). Retrograde tracing confirmed the double innervation of mimetic muscles by the facial and hypoglossal nucleus in both groups. Conclusion: This study showed histologically and physiologically the superior effectiveness of performing IPJG with a collagen-coated PGA conduit in a rat model.

Original languageEnglish (US)
Pages (from-to)70-80
Number of pages11
JournalMicrosurgery
Volume39
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Polyglycolic Acid
Facial Paralysis
Facial Nerve
Collagen
Transplants
Autografts
Action Potentials
Axons
Hypoglossal Nerve
Facial Muscles
Muscles
Control Groups
Nerve Regeneration
Trigeminal Nerve
Myelin Sheath
Surgical Instruments
Ligation

ASJC Scopus subject areas

  • Surgery

Cite this

Niimi, Y., Matsumine, H., Takeuchi, Y., Hironobu, O., Tsunoda, S., Miyata, M., ... Sakurai, H. (2019). A collagen-coated PGA conduit for interpositional-jump graft with end-to-side neurorrhaphy for treating facial nerve paralysis in rat. Microsurgery, 39(1), 70-80. https://doi.org/10.1002/micr.30291

A collagen-coated PGA conduit for interpositional-jump graft with end-to-side neurorrhaphy for treating facial nerve paralysis in rat. / Niimi, Yosuke; Matsumine, Hajime; Takeuchi, Yuichi; Hironobu, Osaki; Tsunoda, Satoshi; Miyata, Mariko; Yamato, Masayuki; Sakurai, Hiroyuki.

In: Microsurgery, Vol. 39, No. 1, 01.01.2019, p. 70-80.

Research output: Contribution to journalArticle

Niimi, Y, Matsumine, H, Takeuchi, Y, Hironobu, O, Tsunoda, S, Miyata, M, Yamato, M & Sakurai, H 2019, 'A collagen-coated PGA conduit for interpositional-jump graft with end-to-side neurorrhaphy for treating facial nerve paralysis in rat', Microsurgery, vol. 39, no. 1, pp. 70-80. https://doi.org/10.1002/micr.30291
Niimi, Yosuke ; Matsumine, Hajime ; Takeuchi, Yuichi ; Hironobu, Osaki ; Tsunoda, Satoshi ; Miyata, Mariko ; Yamato, Masayuki ; Sakurai, Hiroyuki. / A collagen-coated PGA conduit for interpositional-jump graft with end-to-side neurorrhaphy for treating facial nerve paralysis in rat. In: Microsurgery. 2019 ; Vol. 39, No. 1. pp. 70-80.
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