A combination of naturally occurring mutations in North American West Nile virus nonstructural protein genes and in the 3′ untranslated region alters virus phenotype

C. Todd Davis, Sareen E. Galbraith, Shuliu Zhang, Melissa C. Whiteman, Li Li, Richard M. Kinney, Alan Barrett

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We previously reported mutations in North American West Nile viruses (WNVs) with a small-plaque (sp), temperature-sensitive (ts), and/or mouse-attenuated (att) phenotype. Using an infectious clone, site-directed mutations and 3′ untranslated region (3′UTR) exchanges were introduced into the WNV NY99 genome. Characterization of mutants demonstrated that a combination of mutations involving the NS4B protein (E249G) together with either a mutation in the NS5 protein (A804V) or three mutations in the 3′UTR (A10596G, C10774U, A10799G) produced sp, ts, and/or att variants. These results suggested that the discovery of North American WNV-phenotypic variants is rare because of the apparent requirement of concurrent polygenic mutations.

Original languageEnglish (US)
Pages (from-to)6111-6116
Number of pages6
JournalJournal of Virology
Volume81
Issue number11
DOIs
StatePublished - Jun 2007

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West Nile virus
3' Untranslated Regions
3' untranslated regions
Viruses
Phenotype
mutation
phenotype
viruses
Mutation
Proteins
genes
proteins
Temperature
temperature
Clone Cells
Genome
clones
mutants
genome
mice

ASJC Scopus subject areas

  • Immunology

Cite this

A combination of naturally occurring mutations in North American West Nile virus nonstructural protein genes and in the 3′ untranslated region alters virus phenotype. / Davis, C. Todd; Galbraith, Sareen E.; Zhang, Shuliu; Whiteman, Melissa C.; Li, Li; Kinney, Richard M.; Barrett, Alan.

In: Journal of Virology, Vol. 81, No. 11, 06.2007, p. 6111-6116.

Research output: Contribution to journalArticle

Davis, C. Todd ; Galbraith, Sareen E. ; Zhang, Shuliu ; Whiteman, Melissa C. ; Li, Li ; Kinney, Richard M. ; Barrett, Alan. / A combination of naturally occurring mutations in North American West Nile virus nonstructural protein genes and in the 3′ untranslated region alters virus phenotype. In: Journal of Virology. 2007 ; Vol. 81, No. 11. pp. 6111-6116.
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