A comparison of the insulinotropic and the insulin-inhibitory actions of gut peptides on newborn and adult rat islet cells

J. Ishizuka, Pomila Singh, G. H. Greeley, Courtney Townsend, C. W. Cooper, K. Tatemoto, J. C. Thompson

Research output: Contribution to journalArticle

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Abstract

The objective of this study was to examine the release of insulin from cultured islet cells, taken from the pancreas of newborn and adult rats, in response to gastric inhibitory polypeptide (GIP), cholecystokinin-8 (CCK-8), calcitonin gene-related peptide (CGRP), and pancreastatin. GIP (10-9 - 10-7 M) potentiated glucose-stimulated release of insulin in a dose-dependent fashion from both newborn and adult islet cells. CCK-8 (>10-8 M) also increased glucose-stimulated release of insulin from newborn islet cells, however its effect was not significant and not as strong as that observed with adult islet cells. Culture of newborn islet cells for 3 weeks with media containing high concentrations of glucose (16.7 mM) enhanced insulin release in response to CCK-8. CGRP did not affect the release of insulin from newborn islet cells, whereas at 10-10 M, it reduced the release of insulin from adult islet cells by 66 ± 4%. Pancreastatin (10-9 - 10-8 M) did not affect the release of insulin from newborn islet cells when cells were incubated with 4.2 mM glucose, whereas it stimulated the release of insulin from adult islet cells in a dose-dependent fashion. When incubated with 16.7 mM glucose, pancreastatin inhibited the release of insulin from both newborn and adult islet cells. These results indicate that newborn islet cells experience developmental changes which render them responsive to enteric peptides.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalPancreas
Volume3
Issue number1
StatePublished - 1988

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Islets of Langerhans
Insulin
Peptides
Glucose
Gastric Inhibitory Polypeptide
Calcitonin Gene-Related Peptide
Pancreas
Cultured Cells

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

A comparison of the insulinotropic and the insulin-inhibitory actions of gut peptides on newborn and adult rat islet cells. / Ishizuka, J.; Singh, Pomila; Greeley, G. H.; Townsend, Courtney; Cooper, C. W.; Tatemoto, K.; Thompson, J. C.

In: Pancreas, Vol. 3, No. 1, 1988, p. 77-82.

Research output: Contribution to journalArticle

Ishizuka, J, Singh, P, Greeley, GH, Townsend, C, Cooper, CW, Tatemoto, K & Thompson, JC 1988, 'A comparison of the insulinotropic and the insulin-inhibitory actions of gut peptides on newborn and adult rat islet cells', Pancreas, vol. 3, no. 1, pp. 77-82.
Ishizuka, J. ; Singh, Pomila ; Greeley, G. H. ; Townsend, Courtney ; Cooper, C. W. ; Tatemoto, K. ; Thompson, J. C. / A comparison of the insulinotropic and the insulin-inhibitory actions of gut peptides on newborn and adult rat islet cells. In: Pancreas. 1988 ; Vol. 3, No. 1. pp. 77-82.
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