A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Ying Huang, William K. Russell, Wei Wan, Pei Jing Pai, David H. Russell, Wenshe Liu

Research output: Contribution to journalArticle

45 Scopus citations


By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl- l-lysines into one GFPUV protein in E. coli.

Original languageEnglish (US)
Pages (from-to)683-686
Number of pages4
JournalMolecular BioSystems
Issue number4
StatePublished - Mar 26 2010
Externally publishedYes


ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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