A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Ying Huang; William K. Russell; Wei Wan; Pei Jing Pai; David H. Russell; Wenshe Liu

doi:10.1039/b920120c

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Ying Huang
, William K. Russell
, Wei Wan
, Pei Jing Pai
, David H. Russell
, Wenshe Liu

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N^ε-acetyl- l-lysines into one GFP_UV protein in E. coli.

Original language	English (US)
Pages (from-to)	683-686
Number of pages	4
Journal	Molecular BioSystems
Volume	6
Issue number	4
DOIs	https://doi.org/10.1039/b920120c
State	Published - 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Molecular Biology

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10.1039/b920120c

Cite this

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title = "A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli",

abstract = "By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl- l-lysines into one GFPUV protein in E. coli.",

author = "Ying Huang and Russell, \{William K.\} and Wei Wan and Pai, \{Pei Jing\} and Russell, \{David H.\} and Wenshe Liu",

year = "2010",

doi = "10.1039/b920120c",

language = "English (US)",

volume = "6",

pages = "683--686",

journal = "Molecular BioSystems",

issn = "1742-206X",

publisher = "Royal Society of Chemistry",

number = "4",

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TY - JOUR

T1 - A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

AU - Huang, Ying

AU - Russell, William K.

AU - Wan, Wei

AU - Pai, Pei Jing

AU - Russell, David H.

AU - Liu, Wenshe

PY - 2010

Y1 - 2010

N2 - By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl- l-lysines into one GFPUV protein in E. coli.

AB - By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl- l-lysines into one GFPUV protein in E. coli.

UR - https://www.scopus.com/pages/publications/77949757079

UR - https://www.scopus.com/pages/publications/77949757079#tab=citedBy

U2 - 10.1039/b920120c

DO - 10.1039/b920120c

M3 - Article

C2 - 20237646

AN - SCOPUS:77949757079

SN - 1742-206X

VL - 6

SP - 683

EP - 686

JO - Molecular BioSystems

JF - Molecular BioSystems

IS - 4

ER -

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Abstract

ASJC Scopus subject areas

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