A critical role for CD63 in HIV replication and infection of macrophages and cell lines

Hui Chen, Natallia Dziuba, Brian Friedrich, Jana von Lindern, James L. Murray, Daniel R. Rojo, Thomas W. Hodge, William A. O'Brien, Monique Ferguson

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

HIV infection typically involves interaction of Env with CD4 and a chemokine coreceptor, either CCR5 or CXCR4. Other cellular factors supporting HIV replication have also been characterized. We previously demonstrated a role for CD63 in early HIV infection events in macrophages via inhibition by anti-CD63 antibody pretreatment. To confirm the requirement for CD63 in HIV replication, we decreased CD63 expression using CD63-specific short interfering RNAs (siRNA), and showed inhibition of HIV replication in macrophages. Surprisingly, pretreatment with CD63 siRNA not only silenced CD63 expression by 90%, but also inhibited HIV-1 replication in a cultured cell line (U373-MAGI) which had been previously shown to be insensitive to CD63 monoclonal antibody inhibition. Although the anti-CD63 antibody was previously shown to inhibit early HIV infection events only in macrophages, we now show a potential role for CD63 in later HIV replication events in macrophages and cell lines. Further delineation of the role of CD63 in HIV replication may lead to development of novel therapeutic compounds.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalVirology
Volume379
Issue number2
DOIs
StatePublished - Sep 30 2008

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Keywords

  • CD63
  • HIV-1
  • Macrophages
  • siRNA
  • Tetraspanin

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Chen, H., Dziuba, N., Friedrich, B., Lindern, J. V., Murray, J. L., Rojo, D. R., Hodge, T. W., O'Brien, W. A., & Ferguson, M. (2008). A critical role for CD63 in HIV replication and infection of macrophages and cell lines. Virology, 379(2), 191-196. https://doi.org/10.1016/j.virol.2008.06.029