A cytokine-producing profile of anti-type 2 t cells induced in normal mice by treatment with benzoylmesaconine (BEN)

N. Nagata, A. Kanako, M. Kobavashi, David Herndon, R. B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Marked protective effects of CD4+ anti-type 2 Tcells (BEN-Tcells), which were generated in spleens of mice treated with BEN (a non-toxic alkarold extracted from Aconiti tuber), have been shown in thermally injured mice infected with herpes simplex virus type 1. In the present study BEN-Tcells were characterized by their cytokine-producing profile. Purified BEN-Tcells were prepared from mice treated with BEN (Tsumura Co., Tokyo, Japan), as described. The anti-type 2 T cell activity was analyzed by a modified mixed lymphocyte reaction supplemented with T6S cells, a clone of burn-associated CDStype 2 Tcells. Activities of various cytokines were assayed as described previously. Cytokine mRNA expression of BEN-T cells was determined by RT-PCR method. As controls, LB2 cells, obtained from ATCC, and T6S cells were used as authentic Th1 and Th2 cells, respectively. BEN-Tcells (1x106 cells/ml) produced 170 U/ml of IFN-y when they were cultured with anti-CD3 mAb(2.5 ng/ml) for 48 hrs. However, the activities of IL-2. IL-4 and IL-10 were not detected in their culture fluids. IL-2 (160 U/ml) and IFN-y (190 U/ml) were produced by Th1 cells (1x106 cells/ml) stimulated the mAb. Th2 cells (1x106 cells/ml) produced IL4 (80 U/ml) and IL-10 (70 U/ml), but not produce IFN-r and IL-2, after the stimulation. BEN-T cells expressed mRNA for only IFN-r. when Th1 cells expressed for IFN-y mRNA and IL-2 mRNA and Th2 cells expressed for IL-4 mRNA and IL-10 mRNA. These results may suggest that BEN-T cells are a new CD4T cell subset, not belonging to Th1 and Th2 cells.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

cytokines
Cytokines
mice
Th2 Cells
Th1 Cells
T-cells
Messenger RNA
Interleukin-2
cells
Interleukin-4
Interleukin-10
interleukin-2
T-Lymphocytes
interleukin-10
T-lymphocytes
interleukin-4
benzoylmesaconine
Mixed Lymphocyte Culture Test
Lymphocytes
Tokyo

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

A cytokine-producing profile of anti-type 2 t cells induced in normal mice by treatment with benzoylmesaconine (BEN). / Nagata, N.; Kanako, A.; Kobavashi, M.; Herndon, David; Pollard, R. B.; Suzuki, Fujio.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Nagata, N. ; Kanako, A. ; Kobavashi, M. ; Herndon, David ; Pollard, R. B. ; Suzuki, Fujio. / A cytokine-producing profile of anti-type 2 t cells induced in normal mice by treatment with benzoylmesaconine (BEN). In: FASEB Journal. 1996 ; Vol. 10, No. 6.
@article{33bb2f77fc5e4055a8b4150acda482ff,
title = "A cytokine-producing profile of anti-type 2 t cells induced in normal mice by treatment with benzoylmesaconine (BEN)",
abstract = "Marked protective effects of CD4+ anti-type 2 Tcells (BEN-Tcells), which were generated in spleens of mice treated with BEN (a non-toxic alkarold extracted from Aconiti tuber), have been shown in thermally injured mice infected with herpes simplex virus type 1. In the present study BEN-Tcells were characterized by their cytokine-producing profile. Purified BEN-Tcells were prepared from mice treated with BEN (Tsumura Co., Tokyo, Japan), as described. The anti-type 2 T cell activity was analyzed by a modified mixed lymphocyte reaction supplemented with T6S cells, a clone of burn-associated CDStype 2 Tcells. Activities of various cytokines were assayed as described previously. Cytokine mRNA expression of BEN-T cells was determined by RT-PCR method. As controls, LB2 cells, obtained from ATCC, and T6S cells were used as authentic Th1 and Th2 cells, respectively. BEN-Tcells (1x106 cells/ml) produced 170 U/ml of IFN-y when they were cultured with anti-CD3 mAb(2.5 ng/ml) for 48 hrs. However, the activities of IL-2. IL-4 and IL-10 were not detected in their culture fluids. IL-2 (160 U/ml) and IFN-y (190 U/ml) were produced by Th1 cells (1x106 cells/ml) stimulated the mAb. Th2 cells (1x106 cells/ml) produced IL4 (80 U/ml) and IL-10 (70 U/ml), but not produce IFN-r and IL-2, after the stimulation. BEN-T cells expressed mRNA for only IFN-r. when Th1 cells expressed for IFN-y mRNA and IL-2 mRNA and Th2 cells expressed for IL-4 mRNA and IL-10 mRNA. These results may suggest that BEN-T cells are a new CD4T cell subset, not belonging to Th1 and Th2 cells.",
author = "N. Nagata and A. Kanako and M. Kobavashi and David Herndon and Pollard, {R. B.} and Fujio Suzuki",
year = "1996",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - A cytokine-producing profile of anti-type 2 t cells induced in normal mice by treatment with benzoylmesaconine (BEN)

AU - Nagata, N.

AU - Kanako, A.

AU - Kobavashi, M.

AU - Herndon, David

AU - Pollard, R. B.

AU - Suzuki, Fujio

PY - 1996

Y1 - 1996

N2 - Marked protective effects of CD4+ anti-type 2 Tcells (BEN-Tcells), which were generated in spleens of mice treated with BEN (a non-toxic alkarold extracted from Aconiti tuber), have been shown in thermally injured mice infected with herpes simplex virus type 1. In the present study BEN-Tcells were characterized by their cytokine-producing profile. Purified BEN-Tcells were prepared from mice treated with BEN (Tsumura Co., Tokyo, Japan), as described. The anti-type 2 T cell activity was analyzed by a modified mixed lymphocyte reaction supplemented with T6S cells, a clone of burn-associated CDStype 2 Tcells. Activities of various cytokines were assayed as described previously. Cytokine mRNA expression of BEN-T cells was determined by RT-PCR method. As controls, LB2 cells, obtained from ATCC, and T6S cells were used as authentic Th1 and Th2 cells, respectively. BEN-Tcells (1x106 cells/ml) produced 170 U/ml of IFN-y when they were cultured with anti-CD3 mAb(2.5 ng/ml) for 48 hrs. However, the activities of IL-2. IL-4 and IL-10 were not detected in their culture fluids. IL-2 (160 U/ml) and IFN-y (190 U/ml) were produced by Th1 cells (1x106 cells/ml) stimulated the mAb. Th2 cells (1x106 cells/ml) produced IL4 (80 U/ml) and IL-10 (70 U/ml), but not produce IFN-r and IL-2, after the stimulation. BEN-T cells expressed mRNA for only IFN-r. when Th1 cells expressed for IFN-y mRNA and IL-2 mRNA and Th2 cells expressed for IL-4 mRNA and IL-10 mRNA. These results may suggest that BEN-T cells are a new CD4T cell subset, not belonging to Th1 and Th2 cells.

AB - Marked protective effects of CD4+ anti-type 2 Tcells (BEN-Tcells), which were generated in spleens of mice treated with BEN (a non-toxic alkarold extracted from Aconiti tuber), have been shown in thermally injured mice infected with herpes simplex virus type 1. In the present study BEN-Tcells were characterized by their cytokine-producing profile. Purified BEN-Tcells were prepared from mice treated with BEN (Tsumura Co., Tokyo, Japan), as described. The anti-type 2 T cell activity was analyzed by a modified mixed lymphocyte reaction supplemented with T6S cells, a clone of burn-associated CDStype 2 Tcells. Activities of various cytokines were assayed as described previously. Cytokine mRNA expression of BEN-T cells was determined by RT-PCR method. As controls, LB2 cells, obtained from ATCC, and T6S cells were used as authentic Th1 and Th2 cells, respectively. BEN-Tcells (1x106 cells/ml) produced 170 U/ml of IFN-y when they were cultured with anti-CD3 mAb(2.5 ng/ml) for 48 hrs. However, the activities of IL-2. IL-4 and IL-10 were not detected in their culture fluids. IL-2 (160 U/ml) and IFN-y (190 U/ml) were produced by Th1 cells (1x106 cells/ml) stimulated the mAb. Th2 cells (1x106 cells/ml) produced IL4 (80 U/ml) and IL-10 (70 U/ml), but not produce IFN-r and IL-2, after the stimulation. BEN-T cells expressed mRNA for only IFN-r. when Th1 cells expressed for IFN-y mRNA and IL-2 mRNA and Th2 cells expressed for IL-4 mRNA and IL-10 mRNA. These results may suggest that BEN-T cells are a new CD4T cell subset, not belonging to Th1 and Th2 cells.

UR - http://www.scopus.com/inward/record.url?scp=4243356577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4243356577&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:4243356577

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -