A Cytophotometric analysis of the DNA in the nucleus of the giant cell, R-2, in Aplysia

Richard E. Coggeshall, Barbara A. Yaksta, Frank J. Swartz

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

DNA was cytophotometrically measured in Feulgen stained nuclei of R-2, the giant neuron of the abdominal ganglion in Aplysia. The data indicate that the nucleus hag a volume of more than 7 X 106 μ3 in large animals, and contains as much as 75000 times the haploid amount of DNA. To our knowledge, this is the most highly polyploid nucleus yet described. Furthermore, the amount of DNA increases with growth, going from approximately 2000 times the haploid amount in small animals to over 75000 times in large animals. The data suggest that the increase in DNA occurs in increments, each increment having approximately twice the DNA as the one before. Thus we suggest that the increase in DNA in the nucleus of R-2 results from the entire genome replicating without accompanying cell division.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalChromosoma
Volume32
Issue number2
DOIs
StatePublished - Jun 1971
Externally publishedYes

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Aplysia
Giant Cells
DNA
Haploidy
Polyploidy
Ganglia
Cell Division
Genome
Neurons
Growth

ASJC Scopus subject areas

  • Genetics

Cite this

A Cytophotometric analysis of the DNA in the nucleus of the giant cell, R-2, in Aplysia. / Coggeshall, Richard E.; Yaksta, Barbara A.; Swartz, Frank J.

In: Chromosoma, Vol. 32, No. 2, 06.1971, p. 205-212.

Research output: Contribution to journalArticle

Coggeshall, Richard E. ; Yaksta, Barbara A. ; Swartz, Frank J. / A Cytophotometric analysis of the DNA in the nucleus of the giant cell, R-2, in Aplysia. In: Chromosoma. 1971 ; Vol. 32, No. 2. pp. 205-212.
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