A deficiency in DNA repair and DNA-PKcs expression in the radiosensitive BALB/c mouse

Ryuichi Okayasu; Katsutoshi Suetomi; Yongjia Yu; Andy Silver; Joel S. Bedford; Roger Cox; Robert L. Ullrich

A deficiency in DNA repair and DNA-PKcs expression in the radiosensitive BALB/c mouse

Ryuichi Okayasu, Katsutoshi Suetomi, Yongjia Yu, Andy Silver, Joel S. Bedford, Roger Cox, Robert L. Ullrich

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

We have studied the efficiency of DNA double strand break (DSB) rejoining in primary cells from mouse strains that show large differences in in vivo radiosensitivity and tumor susceptibility. Cells from radiosensitive, cancer-prone BALB/c mice showed inefficient end joining of γ ray-induced DSBs as compared with cells from all of the other commonly used strains and F₁ hybrids of C57BL/6 and BALB/c mice. The BALB/c repair phenotype was accompanied by a significantly reduced expression level of DNA-PKcs protein as well as a lowered DNA-PK activity level as compared with the other strains. In conjunction with published reports, these data suggest that natural genetic variation in nonhomologous end joining processes may have a significant impact on the in vivo radiation response of mice.

Original language	English (US)
Pages (from-to)	4342-4345
Number of pages	4
Journal	Cancer Research
Volume	60
Issue number	16
State	Published - Aug 15 2000

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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abstract = "We have studied the efficiency of DNA double strand break (DSB) rejoining in primary cells from mouse strains that show large differences in in vivo radiosensitivity and tumor susceptibility. Cells from radiosensitive, cancer-prone BALB/c mice showed inefficient end joining of γ ray-induced DSBs as compared with cells from all of the other commonly used strains and F1 hybrids of C57BL/6 and BALB/c mice. The BALB/c repair phenotype was accompanied by a significantly reduced expression level of DNA-PKcs protein as well as a lowered DNA-PK activity level as compared with the other strains. In conjunction with published reports, these data suggest that natural genetic variation in nonhomologous end joining processes may have a significant impact on the in vivo radiation response of mice.",

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AU - Okayasu, Ryuichi

AU - Suetomi, Katsutoshi

AU - Yu, Yongjia

AU - Silver, Andy

AU - Bedford, Joel S.

AU - Cox, Roger

AU - Ullrich, Robert L.

PY - 2000/8/15

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A deficiency in DNA repair and DNA-PKcs expression in the radiosensitive BALB/c mouse

Abstract

ASJC Scopus subject areas

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