A T cell receptor transgenic mouse line reactive to a microbiota flagellin, CBir1, was used to define mechanisms of host microbiota homeostasis. Intestinal IgA, but not serum IgA, was found to block mucosal flagellin uptake and systemic T cell activation in mice. Depletion of CD4+CD25+ Tregs decreased IgA+ B cells, total IgA, and CBir1-specific IgA in gut within days. Repletion of T cell-deficient mice with either CD4 +CD25+ or CD4+foxp3+ Tregs restored intestinal IgA to a much greater extent than their reciprocal CD4+ subsets, indicating that Tregs are the major helper cells for IgA responses to microbiota antigens such as flagellin. We propose that the major role of this coordinated Treg-IgA response is to maintain commensalism with the microbiota.
|Number of pages
|Proceedings of the National Academy of Sciences of the United States of America
|Published - Nov 17 2009
- T cells
ASJC Scopus subject areas