A dual role for poly-ADP-ribosylation in spatial memory acquisition after traumatic brain injury in mice involving NAD+ depletion and ribosylation of 14-3-3γ

Margaret A. Satchell, Xiaopeng Zhang, Patrick M. Kochanek, C. Edward Dixon, Larry W. Jenkins, John Melick, Csaba Szabo, Robert S B Clark

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1) is a homeostatic enzyme that paradoxically contributes to disturbances in spatial memory acquisition after traumatic brain injury (TBI) in transgenic mice, thought to be related to depletion of its substrate nicotinamide adenine dinucleotide (NAD+). In this study, systemic administration of the PARP-1 inhibitor 5-iodo-6-amino-1,2-benzopyrone (INH2BP) after TBI preserved brain NAD+ levels and dose-dependently reduced poly-ADP-ribosylation 24 h after injury. While moderate-dose INH2BP improved spatial memory acquisition after TBI; strikingly, both injured- and sham-mice receiving high-dose INH2BP were unable to learn in the Morris-water maze. Poly-ADP-ribosylated peptides identified using a proteomics approach yielded several proteins potentially associated with memory, including structural proteins (tubulin α and β, γ-actin, and α-internexin neuronal intermediate filament protein) and 14-3-3γ. Nuclear poly-ADP-ribosylation of 14-3-3γ was completely inhibited by the dose of INH2BP that produced profound memory disturbances. Thus, partial inhibition of poly-ADP-ribosylation preserves NAD+ and improves functional outcome after TBI, whereas more complete inhibition impairs spatial memory acquisition independent of injury, and is associated with ribosylation of 14-3-3γ.

Original languageEnglish (US)
Pages (from-to)697-708
Number of pages12
JournalJournal of Neurochemistry
Volume85
Issue number3
StatePublished - May 2003
Externally publishedYes

Fingerprint

NAD
Adenosine Diphosphate
Brain
Data storage equipment
Poly(ADP-ribose) Polymerases
14-3-3 Proteins
Intermediate Filament Proteins
Wounds and Injuries
Tubulin
Proteomics
Transgenic Mice
Actins
Proteins
Peptides
5-iodo-6-amino-1,2-benzopyrone
Spatial Memory
Traumatic Brain Injury
Water
Enzymes
Substrates

Keywords

  • 14-3-3
  • 5-iodo-6-amino-1,2-benzopyrone
  • DNA damage
  • Head injury
  • Nicotinamide adenine dinucleotide
  • Poly(ADP-ribose) synthetase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Satchell, M. A., Zhang, X., Kochanek, P. M., Dixon, C. E., Jenkins, L. W., Melick, J., ... Clark, R. S. B. (2003). A dual role for poly-ADP-ribosylation in spatial memory acquisition after traumatic brain injury in mice involving NAD+ depletion and ribosylation of 14-3-3γ. Journal of Neurochemistry, 85(3), 697-708.

A dual role for poly-ADP-ribosylation in spatial memory acquisition after traumatic brain injury in mice involving NAD+ depletion and ribosylation of 14-3-3γ. / Satchell, Margaret A.; Zhang, Xiaopeng; Kochanek, Patrick M.; Dixon, C. Edward; Jenkins, Larry W.; Melick, John; Szabo, Csaba; Clark, Robert S B.

In: Journal of Neurochemistry, Vol. 85, No. 3, 05.2003, p. 697-708.

Research output: Contribution to journalArticle

Satchell, Margaret A. ; Zhang, Xiaopeng ; Kochanek, Patrick M. ; Dixon, C. Edward ; Jenkins, Larry W. ; Melick, John ; Szabo, Csaba ; Clark, Robert S B. / A dual role for poly-ADP-ribosylation in spatial memory acquisition after traumatic brain injury in mice involving NAD+ depletion and ribosylation of 14-3-3γ. In: Journal of Neurochemistry. 2003 ; Vol. 85, No. 3. pp. 697-708.
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