A facile method to synthesize histones with posttranslational modification mimics

Zhiyong U. Wang, Yane Shih Wang, Pei Jing Pai, William Russell, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Using an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

Original languageEnglish (US)
Pages (from-to)5232-5234
Number of pages3
JournalBiochemistry
Volume51
Issue number26
DOIs
StatePublished - Jul 3 2012
Externally publishedYes

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Post Translational Protein Processing
Histones
Lysine
Amino Acyl-tRNA Synthetases
Acetylation
Nucleophiles
Addition reactions
Phosphorylation
Methylation
Sulfhydryl Compounds
Serine
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wang, Z. U., Wang, Y. S., Pai, P. J., Russell, W., Russell, D. H., & Liu, W. R. (2012). A facile method to synthesize histones with posttranslational modification mimics. Biochemistry, 51(26), 5232-5234. https://doi.org/10.1021/bi300535a

A facile method to synthesize histones with posttranslational modification mimics. / Wang, Zhiyong U.; Wang, Yane Shih; Pai, Pei Jing; Russell, William; Russell, David H.; Liu, Wenshe R.

In: Biochemistry, Vol. 51, No. 26, 03.07.2012, p. 5232-5234.

Research output: Contribution to journalArticle

Wang, ZU, Wang, YS, Pai, PJ, Russell, W, Russell, DH & Liu, WR 2012, 'A facile method to synthesize histones with posttranslational modification mimics', Biochemistry, vol. 51, no. 26, pp. 5232-5234. https://doi.org/10.1021/bi300535a
Wang, Zhiyong U. ; Wang, Yane Shih ; Pai, Pei Jing ; Russell, William ; Russell, David H. ; Liu, Wenshe R. / A facile method to synthesize histones with posttranslational modification mimics. In: Biochemistry. 2012 ; Vol. 51, No. 26. pp. 5232-5234.
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