A facile method to synthesize histones with posttranslational modification mimics

Zhiyong U. Wang, Yane Shih Wang, Pei Jing Pai, William K. Russell, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Using an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

Original languageEnglish (US)
Pages (from-to)5232-5234
Number of pages3
JournalBiochemistry
Volume51
Issue number26
DOIs
StatePublished - Jul 3 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Wang, Z. U., Wang, Y. S., Pai, P. J., Russell, W. K., Russell, D. H., & Liu, W. R. (2012). A facile method to synthesize histones with posttranslational modification mimics. Biochemistry, 51(26), 5232-5234. https://doi.org/10.1021/bi300535a