A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli

Wei Wan; Ying Huang; Zhiyong Wang; William K. Russell; Pei Jing Pai; David H. Russell; Wenshe R. Liu

doi:10.1002/anie.201000465

A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli

Wei Wan, Ying Huang, Zhiyong Wang, William K. Russell, Pei Jing Pai, David H. Russell, Wenshe R. Liu

Research output: Contribution to journal › Article › peer-review

186 Scopus citations

Abstract

Two's company: Using a wild-type or evolved PylRS-pylT_UUA pair to suppress ochre mutation and an evolved MjTyrRSMjtRNA_CUA ^Tyr pair to suppress amber mutation, two different noncanonical amino acids (NAAs) have been concomitantly incorporated into one protein in E. coli with high efficiency (see picture, with NAAs 1-4; GFP = green-fluorescent protein). "Chemical equation presented"

Original language	English (US)
Pages (from-to)	3211-3214
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	18
DOIs	https://doi.org/10.1002/anie.201000465
State	Published - Apr 19 2010
Externally published	Yes

Keywords

Amber suppression
Genetic code expansion
Noncanonical amino acids
Protein engineering

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201000465

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abstract = "Two's company: Using a wild-type or evolved PylRS-pylTUUA pair to suppress ochre mutation and an evolved MjTyrRSMjtRNACUA Tyr pair to suppress amber mutation, two different noncanonical amino acids (NAAs) have been concomitantly incorporated into one protein in E. coli with high efficiency (see picture, with NAAs 1-4; GFP = green-fluorescent protein). {"}Chemical equation presented{"}",

keywords = "Amber suppression, Genetic code expansion, Noncanonical amino acids, Protein engineering",

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AU - Wan, Wei

AU - Huang, Ying

AU - Wang, Zhiyong

AU - Russell, William K.

AU - Pai, Pei Jing

AU - Russell, David H.

AU - Liu, Wenshe R.

PY - 2010/4/19

Y1 - 2010/4/19

N2 - Two's company: Using a wild-type or evolved PylRS-pylTUUA pair to suppress ochre mutation and an evolved MjTyrRSMjtRNACUA Tyr pair to suppress amber mutation, two different noncanonical amino acids (NAAs) have been concomitantly incorporated into one protein in E. coli with high efficiency (see picture, with NAAs 1-4; GFP = green-fluorescent protein). "Chemical equation presented"

AB - Two's company: Using a wild-type or evolved PylRS-pylTUUA pair to suppress ochre mutation and an evolved MjTyrRSMjtRNACUA Tyr pair to suppress amber mutation, two different noncanonical amino acids (NAAs) have been concomitantly incorporated into one protein in E. coli with high efficiency (see picture, with NAAs 1-4; GFP = green-fluorescent protein). "Chemical equation presented"

KW - Amber suppression

KW - Genetic code expansion

KW - Noncanonical amino acids

KW - Protein engineering

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JF - Angewandte Chemie - International Edition

IS - 18

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A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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