A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli

Wei Wan, Ying Huang, Zhiyong Wang, William Russell, Pei Jing Pai, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Two's company: Using a wild-type or evolved PylRS-pylTUUA pair to suppress ochre mutation and an evolved MjTyrRSMjtRNACUA Tyr pair to suppress amber mutation, two different noncanonical amino acids (NAAs) have been concomitantly incorporated into one protein in E. coli with high efficiency (see picture, with NAAs 1-4; GFP = green-fluorescent protein). "Chemical equation presented"

Original languageEnglish (US)
Pages (from-to)3211-3214
Number of pages4
JournalAngewandte Chemie - International Edition
Volume49
Issue number18
DOIs
StatePublished - Apr 19 2010
Externally publishedYes

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Escherichia coli
Amino acids
Proteins
Amino Acids
Green Fluorescent Proteins
Industry

Keywords

  • Amber suppression
  • Genetic code expansion
  • Noncanonical amino acids
  • Protein engineering

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli. / Wan, Wei; Huang, Ying; Wang, Zhiyong; Russell, William; Pai, Pei Jing; Russell, David H.; Liu, Wenshe R.

In: Angewandte Chemie - International Edition, Vol. 49, No. 18, 19.04.2010, p. 3211-3214.

Research output: Contribution to journalArticle

Wan, Wei ; Huang, Ying ; Wang, Zhiyong ; Russell, William ; Pai, Pei Jing ; Russell, David H. ; Liu, Wenshe R. / A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli. In: Angewandte Chemie - International Edition. 2010 ; Vol. 49, No. 18. pp. 3211-3214.
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