A fast carrier chromatin immunoprecipitation method applicable to microdissected tissue samples

Haiping Hao, Hester Liu, Gregory Gonye, James S. Schwaber

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Transcriptional regulation studies of CNS neurons are complicated by both cellular diversity and plasticity. Microdissection of specific functionally related populations of neurons can greatly reduce these issues, but typically excludes the use of many technologies due to tissue requirements, such as Chromatin Immunoprecipitation (ChIP), a powerful tool for studying in vivo protein-DNA interactions. We have developed a fast carrier ChIP (Fast CChIP) method for analyzing specific in vivo transcription factor-DNA interactions in as little as 0.2 mm3 brain tissue. Using an antibody against phosphorylated cyclic-AMP response element binding (CREB) protein, we confirmed phospho-CREB (pCREB) binding at the c-fos gene promoter. Then we further demonstrated the applicability of Fast CChIP in determining hypertension-induced pCREB binding at the c-fos gene promoter in the rat nucleus tractus solitarius (NTS), confirming CREB's role in mediating hypertension-induced c-fos expression. This method will be broadly applicable to individual brain nucleus and biopsy/surgical samples.

Original languageEnglish (US)
Pages (from-to)38-42
Number of pages5
JournalJournal of Neuroscience Methods
Issue number1
StatePublished - Jul 15 2008
Externally publishedYes


  • CNS
  • ChIP
  • Chromatin immunoprecipitation
  • Fast CChIP
  • Microdissection
  • Transcription regulation
  • qPCR

ASJC Scopus subject areas

  • General Neuroscience


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