A flow cytometric assay of Fc receptor-mediated phagocytosis

Wolfgang H.T. Schürmann, Mark W. Frampton, Gudrun Schürmann, Norbert J. Roberts, Joan E. Nichols, Jacob N. Finkelstein, Günter Oberdörster, Mark J. Utell

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A flow cytometric assay was developed that specifically measures Fc receptor-mediated phagocytosis independent of other phagocytosis-related receptors. Suspensions of rat alveolar macrophages were incubated with fluorescein isothiocyanate (FITC)labeled polystyrene microspheres and subsequently were analyzed by flow cytometry. Coating of microspheres with bovine serum albumin was used to generate 'neutral' particles demonstrating low phagocytosis by alveolar macrophages (AMs). AM attachment and uptake of these particles was increased tenfold with IgG opsonization. This increase was abolished completely by blocking of AM Fc receptors with IgG. The quenching effect of ethidium bromide (EB) on FITC fluorescence was used for differentiating attached from internalized particles. The assay was standardized and optimized for use in toxicologic studies.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalJournal of Aerosol Medicine: Deposition, Clearance, and Effects in the Lung
Volume10
Issue number1
DOIs
StatePublished - 1997

Keywords

  • Fc receptor
  • alveolar macrophages
  • flow cytometry
  • particles
  • phagocytosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pharmacology (medical)

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    Schürmann, W. H. T., Frampton, M. W., Schürmann, G., Roberts, N. J., Nichols, J. E., Finkelstein, J. N., Oberdörster, G., & Utell, M. J. (1997). A flow cytometric assay of Fc receptor-mediated phagocytosis. Journal of Aerosol Medicine: Deposition, Clearance, and Effects in the Lung, 10(1), 1-8. https://doi.org/10.1089/jam.1997.10.1