A functional alternative splicing mutation in human tryptophan hydroxylase-2

X. Zhang, P. J. Nicholls, G. Laje, T. D. Sotnikova, R. R. Gainetdinov, P. R. Albert, G. Rajkowska, C. A. Stockmeier, M. C. Speer, D. C. Steffens, M. C. Austin, F. J. McMahon, K. R R Krishnan, Mariano Garcia-Blanco, M. G. Caron

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The brain serotonergic system has an essential role in the physiological functions of the central nervous system and dysregulation of serotonin (5-HT) homeostasis has been implicated in many neuropsychiatric disorders. The tryptophan hydroxylase-2 (TPH2) gene is the rate-limiting enzyme in brain 5-HT synthesis, and thus is an ideal candidate gene for understanding the role of dysregulation of brain serotonergic homeostasis. Here, we characterized a common, but functional single-nucleotide polymorphism (SNP rs1386493) in the TPH2 gene, which decreases efficiency of normal RNA splicing, resulting in a truncated TPH2 protein (TPH2-TR) by alternative splicing. TPH2-TR, which lacks TPH2 enzyme activity, dominant-negatively affects full-length TPH2 function, causing reduced 5-HT production. The predicted mRNA for TPH2-TR is present in postmortem brain of rs1386493 carriers. The rs13864923 variant does not appear to be overrepresented in either global or multiplex depression cohorts. However, in combination with other gene variants linked to 5-HT homeostasis, this variant may exhibit important epistatic influences.

Original languageEnglish (US)
Pages (from-to)1169-1176
Number of pages8
JournalMolecular Psychiatry
Volume16
Issue number12
DOIs
StatePublished - Dec 2011
Externally publishedYes

Fingerprint

Tryptophan Hydroxylase
Alternative Splicing
Mutation
Serotonin
Homeostasis
Brain
Genes
Single Nucleotide Polymorphism
RNA Splicing
human TPH2 protein
Enzymes
Central Nervous System
Messenger RNA

Keywords

  • alternative splicing
  • serotonin (5-HT)
  • SNP
  • TPH2

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

Cite this

Zhang, X., Nicholls, P. J., Laje, G., Sotnikova, T. D., Gainetdinov, R. R., Albert, P. R., ... Caron, M. G. (2011). A functional alternative splicing mutation in human tryptophan hydroxylase-2. Molecular Psychiatry, 16(12), 1169-1176. https://doi.org/10.1038/mp.2010.99

A functional alternative splicing mutation in human tryptophan hydroxylase-2. / Zhang, X.; Nicholls, P. J.; Laje, G.; Sotnikova, T. D.; Gainetdinov, R. R.; Albert, P. R.; Rajkowska, G.; Stockmeier, C. A.; Speer, M. C.; Steffens, D. C.; Austin, M. C.; McMahon, F. J.; Krishnan, K. R R; Garcia-Blanco, Mariano; Caron, M. G.

In: Molecular Psychiatry, Vol. 16, No. 12, 12.2011, p. 1169-1176.

Research output: Contribution to journalArticle

Zhang, X, Nicholls, PJ, Laje, G, Sotnikova, TD, Gainetdinov, RR, Albert, PR, Rajkowska, G, Stockmeier, CA, Speer, MC, Steffens, DC, Austin, MC, McMahon, FJ, Krishnan, KRR, Garcia-Blanco, M & Caron, MG 2011, 'A functional alternative splicing mutation in human tryptophan hydroxylase-2', Molecular Psychiatry, vol. 16, no. 12, pp. 1169-1176. https://doi.org/10.1038/mp.2010.99
Zhang X, Nicholls PJ, Laje G, Sotnikova TD, Gainetdinov RR, Albert PR et al. A functional alternative splicing mutation in human tryptophan hydroxylase-2. Molecular Psychiatry. 2011 Dec;16(12):1169-1176. https://doi.org/10.1038/mp.2010.99
Zhang, X. ; Nicholls, P. J. ; Laje, G. ; Sotnikova, T. D. ; Gainetdinov, R. R. ; Albert, P. R. ; Rajkowska, G. ; Stockmeier, C. A. ; Speer, M. C. ; Steffens, D. C. ; Austin, M. C. ; McMahon, F. J. ; Krishnan, K. R R ; Garcia-Blanco, Mariano ; Caron, M. G. / A functional alternative splicing mutation in human tryptophan hydroxylase-2. In: Molecular Psychiatry. 2011 ; Vol. 16, No. 12. pp. 1169-1176.
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