A functional isolated pituitary preparation

M. B. Allen, G. Greeley, V. B. Mahesh

    Research output: Contribution to journalArticle

    Abstract

    Noncastrate and ovariectomized rats were killed at 3, 14, 21, and 50 days after stalk section. Blood was assayed for follicle stimulating hormone (FSH), luteinizing hormone (LH), and prolactin, with use of the respective double antibody radioimmunoassays. A dose of exogenous LH RH that would restore the functional activity and evoke an adequate gonadotrophin response was determined. Fourteen days after stalk section, LH RH in varying doses (0.4, 2.0, 10.0, 50.0 ng) was injected 3 times per day for 6 days. On day 21, rats were killed 30 min after a final injection of LH RH. The baseline level of LH in untreated rats was 4 ng/ml. After 0.4 ng LH RH, no change was recorded; after 2.0 ng LH RH, serum LH level was 60 ng/ml; when 10 ng, LH level was 100 ng/ml; and with a dose of 50 ng, the serum concentration was 160 ng/ml. A parallel response was recorded for FSH. These responses indicate that the remaining gonadotrophs can adequately respond to exogenous LH RH.

    Original languageEnglish (US)
    Pages (from-to)461-462
    Number of pages2
    JournalSurgical Forum
    VolumeVol. 25
    StatePublished - Jan 1 1974

    ASJC Scopus subject areas

    • Surgery

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