A general synthetic method toward uridylylated picornavirus VPg proteins

Gerbrand J. Van der Heden van Noort; Catherine H. Schein; Herman S. Overkleeft; Gijsbert A. Van der Marel; Dmitri V. Filippov

doi:10.1002/psc.2508

A general synthetic method toward uridylylated picornavirus VPg proteins

Gerbrand J. Van der Heden van Noort, Catherine H. Schein, Herman S. Overkleeft, Gijsbert A. Van der Marel, Dmitri V. Filippov

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Small proteins called viral protein genome-linked (VPg), attached to the 5′-end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase-based approach.

Original language	English (US)
Pages (from-to)	333-336
Number of pages	4
Journal	Journal of Peptide Science
Volume	19
Issue number	6
DOIs	https://doi.org/10.1002/psc.2508
State	Published - Jun 2013

Keywords

On-line peptide synthesis
Post-translational modification
Uridylylation
Viral nucleoprotein

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Organic Chemistry

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10.1002/psc.2508

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title = "A general synthetic method toward uridylylated picornavirus VPg proteins",

abstract = "Small proteins called viral protein genome-linked (VPg), attached to the 5′-end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase-based approach.",

keywords = "On-line peptide synthesis, Post-translational modification, Uridylylation, Viral nucleoprotein",

author = "{Van der Heden van Noort}, {Gerbrand J.} and Schein, {Catherine H.} and Overkleeft, {Herman S.} and {Van der Marel}, {Gijsbert A.} and Filippov, {Dmitri V.}",

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AU - Van der Heden van Noort, Gerbrand J.

AU - Schein, Catherine H.

AU - Overkleeft, Herman S.

AU - Van der Marel, Gijsbert A.

AU - Filippov, Dmitri V.

PY - 2013/6

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AB - Small proteins called viral protein genome-linked (VPg), attached to the 5′-end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase-based approach.

KW - On-line peptide synthesis

KW - Post-translational modification

KW - Uridylylation

KW - Viral nucleoprotein

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JO - Journal of Peptide Science

JF - Journal of Peptide Science

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ER -

A general synthetic method toward uridylylated picornavirus VPg proteins

Abstract

Keywords

ASJC Scopus subject areas

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