Abstract
Using the amber suppression approach, Nϵ-(4-azidobenzoxycarbonyl)-δ,ϵ-dehydrolysine, an allysine precursor is genetically encoded in E. coli. Its genetic incorporation followed by two sequential biocompatible reactions allows convenient synthesis of proteins with site-specific lysine dimethylation. Using this approach, dimethyl-histone H3 and p53 proteins have been synthesized and used to probe functions of epigenetic enzymes including histone demethylase LSD1 and histone acetyltransferase Tip60. We confirmed that LSD1 is catalytically active toward H3K4me2 and H3K9me2 but inert toward H3K36me2, and methylation at p53 K372 directly activates Tip60 for its catalyzed acetylation at p53 K120.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 212-216 |
| Number of pages | 5 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 56 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2017 |
| Externally published | Yes |
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Keywords
- allysine
- amber suppression
- dimethyllysine
- genetic code expansion
- lysine dimethylation
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)
Cite this
A Genetically Encoded Allysine for the Synthesis of Proteins with Site-Specific Lysine Dimethylation. / Wang, Zhipeng A.; Zeng, Yu; Kurra, Yadagiri; Wang, Xin; Tharp, Jeffery M.; Vatansever, Erol C.; Hsu, Willie; Dai, Susie; Fang, Xinqiang; Liu, Wenshe R.
In: Angewandte Chemie - International Edition, Vol. 56, No. 1, 2017, p. 212-216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Genetically Encoded Allysine for the Synthesis of Proteins with Site-Specific Lysine Dimethylation
AU - Wang, Zhipeng A.
AU - Zeng, Yu
AU - Kurra, Yadagiri
AU - Wang, Xin
AU - Tharp, Jeffery M.
AU - Vatansever, Erol C.
AU - Hsu, Willie
AU - Dai, Susie
AU - Fang, Xinqiang
AU - Liu, Wenshe R.
PY - 2017
Y1 - 2017
N2 - Using the amber suppression approach, Nϵ-(4-azidobenzoxycarbonyl)-δ,ϵ-dehydrolysine, an allysine precursor is genetically encoded in E. coli. Its genetic incorporation followed by two sequential biocompatible reactions allows convenient synthesis of proteins with site-specific lysine dimethylation. Using this approach, dimethyl-histone H3 and p53 proteins have been synthesized and used to probe functions of epigenetic enzymes including histone demethylase LSD1 and histone acetyltransferase Tip60. We confirmed that LSD1 is catalytically active toward H3K4me2 and H3K9me2 but inert toward H3K36me2, and methylation at p53 K372 directly activates Tip60 for its catalyzed acetylation at p53 K120.
AB - Using the amber suppression approach, Nϵ-(4-azidobenzoxycarbonyl)-δ,ϵ-dehydrolysine, an allysine precursor is genetically encoded in E. coli. Its genetic incorporation followed by two sequential biocompatible reactions allows convenient synthesis of proteins with site-specific lysine dimethylation. Using this approach, dimethyl-histone H3 and p53 proteins have been synthesized and used to probe functions of epigenetic enzymes including histone demethylase LSD1 and histone acetyltransferase Tip60. We confirmed that LSD1 is catalytically active toward H3K4me2 and H3K9me2 but inert toward H3K36me2, and methylation at p53 K372 directly activates Tip60 for its catalyzed acetylation at p53 K120.
KW - allysine
KW - amber suppression
KW - dimethyllysine
KW - genetic code expansion
KW - lysine dimethylation
UR - http://www.scopus.com/inward/record.url?scp=85006253017&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006253017&partnerID=8YFLogxK
U2 - 10.1002/anie.201609452
DO - 10.1002/anie.201609452
M3 - Article
C2 - 27910233
AN - SCOPUS:85006253017
VL - 56
SP - 212
EP - 216
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 1
ER -