A genetically encoded photocaged Nε-methyl-l-lysine

Yane Shih Wang, Bo Wu, Zhiyong Wang, Ying Huang, Wei Wan, William Russell, Pei Jing Pai, Yin N. Moe, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A photocaged Nε-methyl-l-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover Nε-methyl-l-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

Original languageEnglish (US)
Pages (from-to)1557-1560
Number of pages4
JournalMolecular BioSystems
Volume6
Issue number9
DOIs
StatePublished - Sep 2010
Externally publishedYes

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Lysine
Amino Acyl-tRNA Synthetases
Terminator Codon
Photolysis
Protein Biosynthesis
Escherichia coli
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Wang, Y. S., Wu, B., Wang, Z., Huang, Y., Wan, W., Russell, W., ... Liu, W. R. (2010). A genetically encoded photocaged Nε-methyl-l-lysine. Molecular BioSystems, 6(9), 1557-1560. https://doi.org/10.1039/c002155e

A genetically encoded photocaged Nε-methyl-l-lysine. / Wang, Yane Shih; Wu, Bo; Wang, Zhiyong; Huang, Ying; Wan, Wei; Russell, William; Pai, Pei Jing; Moe, Yin N.; Russell, David H.; Liu, Wenshe R.

In: Molecular BioSystems, Vol. 6, No. 9, 09.2010, p. 1557-1560.

Research output: Contribution to journalArticle

Wang, YS, Wu, B, Wang, Z, Huang, Y, Wan, W, Russell, W, Pai, PJ, Moe, YN, Russell, DH & Liu, WR 2010, 'A genetically encoded photocaged Nε-methyl-l-lysine', Molecular BioSystems, vol. 6, no. 9, pp. 1557-1560. https://doi.org/10.1039/c002155e
Wang, Yane Shih ; Wu, Bo ; Wang, Zhiyong ; Huang, Ying ; Wan, Wei ; Russell, William ; Pai, Pei Jing ; Moe, Yin N. ; Russell, David H. ; Liu, Wenshe R. / A genetically encoded photocaged Nε-methyl-l-lysine. In: Molecular BioSystems. 2010 ; Vol. 6, No. 9. pp. 1557-1560.
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