A genetically encoded photocaged Nε-methyl-l-lysine

Yane Shih Wang, Bo Wu, Zhiyong Wang, Ying Huang, Wei Wan, William K. Russell, Pei Jing Pai, Yin N. Moe, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticle

58 Scopus citations


A photocaged Nε-methyl-l-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover Nε-methyl-l-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

Original languageEnglish (US)
Pages (from-to)1557-1560
Number of pages4
JournalMolecular BioSystems
Issue number9
StatePublished - Sep 1 2010
Externally publishedYes


ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Wang, Y. S., Wu, B., Wang, Z., Huang, Y., Wan, W., Russell, W. K., Pai, P. J., Moe, Y. N., Russell, D. H., & Liu, W. R. (2010). A genetically encoded photocaged Nε-methyl-l-lysine. Molecular BioSystems, 6(9), 1557-1560. https://doi.org/10.1039/c002155e