A genetically immortalized human stem cell line: A promising new tool for Alzheimer’s disease therapy

Nicha Puangmalai, Alyma Somani, Wipawan Thangnipon, Clive Ballard, Martin Broadstock

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Amyloid-β peptides and hyper-phosphorylated tau are the main pathological hallmarks of Alzheimer’s disease (AD). Given the recent failure of several large-scale clinical trials and the lack of disease-modifying pharmacological treatments, there is an urgent need to develop alternative therapies. A clinical grade human CTX0E03 neural stem cell line has recently passed phase I trials in people with stroke. However, this cell line has not been investigated in other neurodegenerative disorders. This study investigates the survival of CTX0E03 cells under conditions based on the underlying AD pathology. Cell viability assays showed a concentration dependence of this cell line to the toxic effects of Aβ1-42, but not Aβ1-40, and okadaic acid, a phosphatase 2A inhibitor. Notably, CTX0E03 cell line displayed toxicity at concentrations significantly higher than both rat neural stem cells and those previously reported for primary cultures. These results suggest CTX0E03 cells could be developed for clinical trials in AD patients.

Original languageEnglish (US)
Pages (from-to)1135-1144
Number of pages10
JournalEXCLI Journal
Volume14
DOIs
StatePublished - Oct 21 2015
Externally publishedYes

Keywords

  • Alzheimer’s disease
  • Cell viability
  • CTX0E03 cell line
  • Neural stem cell
  • Okadaic acid
  • Oligomeric amyloid-β

ASJC Scopus subject areas

  • Molecular Medicine
  • Animal Science and Zoology
  • Pharmacology
  • Drug Discovery

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