A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae.

G. F. Casperson, N. Walker, A. R. Brasier, H. R. Bourne

Research output: Contribution to journalArticle

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Abstract

Adenylate cyclase in particulate extracts of Saccharomyces cerevisiae utilized either MnATP or MgATP as substrate. A mutation in the CYR1 gene, which codes for the catalytic unit of yeast adenylate cyclase (Matsumoto, K., Uno, I., and Ishikawa, T. (1983) Cell 32, 417-423), eliminated utilization of both MgATP and MnATP, indicating that a single enzyme was responsible for both activities. GTP and guanylyl-5'-imidodiphosphate stimulated yeast adenylate cyclase, while a GDP analog, guanosine-5'-O-(2-thiodiphosphate), competitively inhibited this stimulation. Thermal inactivation studies distinguished putative guanine-nucleotide regulatory protein (N) from the catalytic unit (C) of yeast adenylate cyclase. Yeast N, which conferred guanine nucleotide regulation and the ability to utilize MgATP on yeast C, was quickly inactivated by incubation of particulate extracts at 30 degrees C. In contrast, yeast C, which apparently utilized MnATP as substrate in the absence of a functional N protein, resisted inactivation at 30 degrees C. These observations suggested that physically distinct protein components mediated the catalytic activity of yeast adenylate cyclase and its regulation by guanine nucleotides. These findings indicate a striking homology between the adenylate cyclase systems of S. cerevisiae and those of vertebrate cells.

Original languageEnglish (US)
Pages (from-to)7911-7914
Number of pages4
JournalJournal of Biological Chemistry
Volume258
Issue number13
StatePublished - Jul 10 1983
Externally publishedYes

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Guanine Nucleotides
Adenylyl Cyclases
Yeast
Saccharomyces cerevisiae
Yeasts
Adenosine Triphosphate
Guanylyl Imidodiphosphate
Guanosine Triphosphate
GTP-Binding Proteins
Vertebrates
Substrates
Proteins
Hot Temperature
Catalyst activity
Mutation
Genes
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Casperson, G. F., Walker, N., Brasier, A. R., & Bourne, H. R. (1983). A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry, 258(13), 7911-7914.

A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae. / Casperson, G. F.; Walker, N.; Brasier, A. R.; Bourne, H. R.

In: Journal of Biological Chemistry, Vol. 258, No. 13, 10.07.1983, p. 7911-7914.

Research output: Contribution to journalArticle

Casperson, GF, Walker, N, Brasier, AR & Bourne, HR 1983, 'A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae.', Journal of Biological Chemistry, vol. 258, no. 13, pp. 7911-7914.
Casperson GF, Walker N, Brasier AR, Bourne HR. A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry. 1983 Jul 10;258(13):7911-7914.
Casperson, G. F. ; Walker, N. ; Brasier, A. R. ; Bourne, H. R. / A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 13. pp. 7911-7914.
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