Abstract
A new high pressure liquid chromatography-radioimmunoassay method is described for the measurement of cholecystokinin-8 (CCK-8) and CCK-33/39 in plasma. The plasma levels of CCK-8-sulfate, CCK-8-desulfate, and CCK-33/39 were measured during the infusion of intraduodenal fat. It is found that the CCK-33/39 levels are almost three times as high as the CCK-8-sulfate level on a molar basis in peripheral fat-stimulated dog plasma. These findings are in agreement with the observation by T. Sakamoto and colleagues that CCK-8 is inactivated on hepatic transit while CCK-33 is not affected. Interestingly, we have also found significant levels of the biologically inactive form of CCK-8.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1431-1438 |
| Number of pages | 8 |
| Journal | Journal of Liquid Chromatography |
| Volume | 10 |
| Issue number | 7 |
| State | Published - 1987 |
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ASJC Scopus subject areas
- Molecular Medicine
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A high pressure liquid chromatography-radioimmunoassay method for measurement of cholecystokinin-8 and cholecystokinin-33/39 in plasma. / Draviam, E. J.; Greeley, G. H.; Beauchamp, R. D.; Thompson, J. C.
In: Journal of Liquid Chromatography, Vol. 10, No. 7, 1987, p. 1431-1438.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A high pressure liquid chromatography-radioimmunoassay method for measurement of cholecystokinin-8 and cholecystokinin-33/39 in plasma
AU - Draviam, E. J.
AU - Greeley, G. H.
AU - Beauchamp, R. D.
AU - Thompson, J. C.
PY - 1987
Y1 - 1987
N2 - A new high pressure liquid chromatography-radioimmunoassay method is described for the measurement of cholecystokinin-8 (CCK-8) and CCK-33/39 in plasma. The plasma levels of CCK-8-sulfate, CCK-8-desulfate, and CCK-33/39 were measured during the infusion of intraduodenal fat. It is found that the CCK-33/39 levels are almost three times as high as the CCK-8-sulfate level on a molar basis in peripheral fat-stimulated dog plasma. These findings are in agreement with the observation by T. Sakamoto and colleagues that CCK-8 is inactivated on hepatic transit while CCK-33 is not affected. Interestingly, we have also found significant levels of the biologically inactive form of CCK-8.
AB - A new high pressure liquid chromatography-radioimmunoassay method is described for the measurement of cholecystokinin-8 (CCK-8) and CCK-33/39 in plasma. The plasma levels of CCK-8-sulfate, CCK-8-desulfate, and CCK-33/39 were measured during the infusion of intraduodenal fat. It is found that the CCK-33/39 levels are almost three times as high as the CCK-8-sulfate level on a molar basis in peripheral fat-stimulated dog plasma. These findings are in agreement with the observation by T. Sakamoto and colleagues that CCK-8 is inactivated on hepatic transit while CCK-33 is not affected. Interestingly, we have also found significant levels of the biologically inactive form of CCK-8.
UR - http://www.scopus.com/inward/record.url?scp=0023273617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023273617&partnerID=8YFLogxK
M3 - Article
VL - 10
SP - 1431
EP - 1438
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 7
ER -