A high pressure liquid chromatography-radioimmunoassay method for measurement of cholecystokinin-8 and cholecystokinin-33/39 in plasma

E. J. Draviam, G. H. Greeley, R. D. Beauchamp, J. C. Thompson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new high pressure liquid chromatography-radioimmunoassay method is described for the measurement of cholecystokinin-8 (CCK-8) and CCK-33/39 in plasma. The plasma levels of CCK-8-sulfate, CCK-8-desulfate, and CCK-33/39 were measured during the infusion of intraduodenal fat. It is found that the CCK-33/39 levels are almost three times as high as the CCK-8-sulfate level on a molar basis in peripheral fat-stimulated dog plasma. These findings are in agreement with the observation by T. Sakamoto and colleagues that CCK-8 is inactivated on hepatic transit while CCK-33 is not affected. Interestingly, we have also found significant levels of the biologically inactive form of CCK-8.

Original languageEnglish (US)
Pages (from-to)1431-1438
Number of pages8
JournalJournal of Liquid Chromatography
Volume10
Issue number7
StatePublished - 1987

Fingerprint

High pressure liquid chromatography
Cholecystokinin
Radioimmunoassay
High Pressure Liquid Chromatography
Plasmas
Sulfates
Fats
Observation
cholecystokinin 8
cholecystokinin 39
Dogs
Liver

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

A high pressure liquid chromatography-radioimmunoassay method for measurement of cholecystokinin-8 and cholecystokinin-33/39 in plasma. / Draviam, E. J.; Greeley, G. H.; Beauchamp, R. D.; Thompson, J. C.

In: Journal of Liquid Chromatography, Vol. 10, No. 7, 1987, p. 1431-1438.

Research output: Contribution to journalArticle

Draviam, E. J. ; Greeley, G. H. ; Beauchamp, R. D. ; Thompson, J. C. / A high pressure liquid chromatography-radioimmunoassay method for measurement of cholecystokinin-8 and cholecystokinin-33/39 in plasma. In: Journal of Liquid Chromatography. 1987 ; Vol. 10, No. 7. pp. 1431-1438.
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