A higher plant mitochondrial homologue of the yeast m-AAA protease

Molecular cloning, localization, and putative function

Marta Kolodziejczak, Anna Kolaczkowska, Bartosz Szczesny, Adam Urantowka, Carina Knorpp, Jan Kieleczawa, Hanna Janska

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Mitochondrial AAA metalloproteases play a fundamental role in mitochondrial biogenesis and function. They have been identified in yeast and animals but not yet in plants. This work describes the isolation and sequence analysis of the full-length cDNA from the pea (Pisum sativum) with significant homology to the yeast matrix AAA (m-AAA) protease. The product of this clone was imported into isolated pea mitochondria where it was processed to its mature form (PsFtsH). We have shown that the central region of PsFtsH containing the chaperone domain is exposed to the matrix space. Furthermore, we have demonstrated that the pea protease can complement respiration deficiency in the yta10 and/or yta12 null yeast mutants, indicating that the plant protein can compensate for the loss of at least some of the important m-AAA functions in yeast. Based on biochemical experiments using isolated pea mitochondria, we propose that PsFtsH-like m-AAA is involved in the accumulation of the subunit 9 of the ATP synthase in the mitochondrial membrane.

Original languageEnglish (US)
Pages (from-to)43792-43798
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number46
DOIs
StatePublished - Nov 15 2002
Externally publishedYes

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Cloning
Peas
Molecular Cloning
Yeast
Peptide Hydrolases
Yeasts
Mitochondria
Plant Proteins
Metalloproteases
Mitochondrial Membranes
Organelle Biogenesis
Animals
Complementary DNA
Adenosine Triphosphate
Sequence Analysis
Membranes
Respiration
Clone Cells
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

A higher plant mitochondrial homologue of the yeast m-AAA protease : Molecular cloning, localization, and putative function. / Kolodziejczak, Marta; Kolaczkowska, Anna; Szczesny, Bartosz; Urantowka, Adam; Knorpp, Carina; Kieleczawa, Jan; Janska, Hanna.

In: Journal of Biological Chemistry, Vol. 277, No. 46, 15.11.2002, p. 43792-43798.

Research output: Contribution to journalArticle

Kolodziejczak, Marta ; Kolaczkowska, Anna ; Szczesny, Bartosz ; Urantowka, Adam ; Knorpp, Carina ; Kieleczawa, Jan ; Janska, Hanna. / A higher plant mitochondrial homologue of the yeast m-AAA protease : Molecular cloning, localization, and putative function. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 46. pp. 43792-43798.
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