A Highly Structured, Nuclease-Resistant, Noncoding RNA Produced by Flaviviruses Is Required for Pathogenicity

Gorben P. Pijlman, Anneke Funk, Natasha Kondratieva, Jason Leung, Shessy Torres, Lieke van der Aa, Wen Jun Liu, Ann C. Palmenberg, Pei-Yong Shi, Roy A. Hall, Alexander A. Khromykh

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Viral noncoding RNAs have been shown to play an important role in virus-host interplay to facilitate virus replication. We report that members of the genus Flavivirus, a large group of medically important encephalitic RNA viruses, produce a unique and highly structured noncoding RNA of 0.3-0.5 kb derived from the 3′ untranslated region of the viral genome. Using West Nile virus as a model, we show that this subgenomic RNA is a product of incomplete degradation of viral genomic RNA by cellular ribonucleases. Highly conserved RNA structures located at the beginning of the 3′ untranslated region render this RNA resistant to nucleases, and the resulting subgenomic RNA product is essential for virus-induced cytopathicity and pathogenicity. Thus, flaviviruses evolved a unique strategy to generate a noncoding RNA product that allows them to kill the host more efficiently.

Original languageEnglish (US)
Pages (from-to)579-591
Number of pages13
JournalCell Host and Microbe
Volume4
Issue number6
DOIs
StatePublished - Dec 11 2008
Externally publishedYes

Fingerprint

Flavivirus
Untranslated RNA
Virulence
RNA
Viral RNA
3' Untranslated Regions
Viruses
West Nile virus
Viral Genome
RNA Viruses
Ribonucleases
Virus Replication

Keywords

  • MICROBIO
  • RNA

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

Pijlman, G. P., Funk, A., Kondratieva, N., Leung, J., Torres, S., van der Aa, L., ... Khromykh, A. A. (2008). A Highly Structured, Nuclease-Resistant, Noncoding RNA Produced by Flaviviruses Is Required for Pathogenicity. Cell Host and Microbe, 4(6), 579-591. https://doi.org/10.1016/j.chom.2008.10.007

A Highly Structured, Nuclease-Resistant, Noncoding RNA Produced by Flaviviruses Is Required for Pathogenicity. / Pijlman, Gorben P.; Funk, Anneke; Kondratieva, Natasha; Leung, Jason; Torres, Shessy; van der Aa, Lieke; Liu, Wen Jun; Palmenberg, Ann C.; Shi, Pei-Yong; Hall, Roy A.; Khromykh, Alexander A.

In: Cell Host and Microbe, Vol. 4, No. 6, 11.12.2008, p. 579-591.

Research output: Contribution to journalArticle

Pijlman, GP, Funk, A, Kondratieva, N, Leung, J, Torres, S, van der Aa, L, Liu, WJ, Palmenberg, AC, Shi, P-Y, Hall, RA & Khromykh, AA 2008, 'A Highly Structured, Nuclease-Resistant, Noncoding RNA Produced by Flaviviruses Is Required for Pathogenicity', Cell Host and Microbe, vol. 4, no. 6, pp. 579-591. https://doi.org/10.1016/j.chom.2008.10.007
Pijlman, Gorben P. ; Funk, Anneke ; Kondratieva, Natasha ; Leung, Jason ; Torres, Shessy ; van der Aa, Lieke ; Liu, Wen Jun ; Palmenberg, Ann C. ; Shi, Pei-Yong ; Hall, Roy A. ; Khromykh, Alexander A. / A Highly Structured, Nuclease-Resistant, Noncoding RNA Produced by Flaviviruses Is Required for Pathogenicity. In: Cell Host and Microbe. 2008 ; Vol. 4, No. 6. pp. 579-591.
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