A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis

Sunanda Margrett Williams, Anu V. Chandran, Mahalingam S. Vijayabaskar, Sourav Roy, Hemalatha Balaram, Saraswathi Vishveshwara, Mamannamana Vijayan, Dipankar Chatterji

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: DNA-binding protein from starved cells (Dps) are nano-compartments that can oxidize and store iron rendering protection from free radicals. Results: A histidine-aspartate ionic cluster in mycobaterial Dps2 modulates the rate of iron entry and exit in these proteins. Conclusion: Substitutions that disrupt the cluster interface alter the iron uptake/release properties with localized structural changes. Significance: Identifying important gating residues can help in designing nano-delivery vehicles.

Original languageEnglish (US)
Pages (from-to)11042-11058
Number of pages17
JournalJournal of Biological Chemistry
Volume289
Issue number16
DOIs
StatePublished - Apr 18 2014
Externally publishedYes

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Mycobacterium smegmatis
Histidine
Aspartic Acid
Iron
DNA-Binding Proteins
Free Radicals
Substitution reactions
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Williams, S. M., Chandran, A. V., Vijayabaskar, M. S., Roy, S., Balaram, H., Vishveshwara, S., ... Chatterji, D. (2014). A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis. Journal of Biological Chemistry, 289(16), 11042-11058. https://doi.org/10.1074/jbc.M113.524421

A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis. / Williams, Sunanda Margrett; Chandran, Anu V.; Vijayabaskar, Mahalingam S.; Roy, Sourav; Balaram, Hemalatha; Vishveshwara, Saraswathi; Vijayan, Mamannamana; Chatterji, Dipankar.

In: Journal of Biological Chemistry, Vol. 289, No. 16, 18.04.2014, p. 11042-11058.

Research output: Contribution to journalArticle

Williams, SM, Chandran, AV, Vijayabaskar, MS, Roy, S, Balaram, H, Vishveshwara, S, Vijayan, M & Chatterji, D 2014, 'A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis', Journal of Biological Chemistry, vol. 289, no. 16, pp. 11042-11058. https://doi.org/10.1074/jbc.M113.524421
Williams SM, Chandran AV, Vijayabaskar MS, Roy S, Balaram H, Vishveshwara S et al. A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis. Journal of Biological Chemistry. 2014 Apr 18;289(16):11042-11058. https://doi.org/10.1074/jbc.M113.524421
Williams, Sunanda Margrett ; Chandran, Anu V. ; Vijayabaskar, Mahalingam S. ; Roy, Sourav ; Balaram, Hemalatha ; Vishveshwara, Saraswathi ; Vijayan, Mamannamana ; Chatterji, Dipankar. / A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 16. pp. 11042-11058.
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