A human gastric cancer cell line possesses a functional receptor for gastrin-releasing peptide

Richard J. Bold, Hong Jin Kim, Jin Ishizuka, Courtney M. Townsend, James C. Thompson

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Bombesin (BBS) exhibits diverse biological functions including those of neurotransmitter, regulator of gastrointestinal hormone release, and mitogen. Gastrin-releasing peptide (GRP, the mammalian equivalent of BBS) is found in mucosal cells of the gastric fundus and antrum. We determined whether a human gastric cancer cell line (SIIA) expresses a functional GRP-receptor (GRP-R). BBS increased intracellular calcium ([Ca2+](i)), and a specific GRP-R antagonist, ([D-Phe6, Des-Mef14]-BBS (6-14)-ethylamide), blocked BBS- induced increase in [Ca2+](i). SIIA cells possess GRP-R mRNA by reverse transcriptase-PCR. Furthermore, these cells possess an 80-kDa cell surface protein that specifically binds BBS with two high-binding affinities (K(d1) = 0.6 nM, K(d2) = 6.7 nM). These findings indicate that SIIA cells possess a GRP-R that is capable of physiological signal transduction, though the cellular response remains unknown.

Original languageEnglish (US)
Pages (from-to)12-17
Number of pages6
JournalCancer Investigation
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Bombesin
  • Gastric cancer
  • Gastrin-releasing peptide
  • Signal transduction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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